Dendritic cells (DCs) transfected with a recombinant adenovirus carrying chlamydial major outer membrane protein antigen elicit protective immune responses against genital tract challenge infection.
Dendritic cells (DCs) transfected with a recombinant adenovirus carrying chlamydial major outer membrane protein antigen elicit protective immune responses against genital tract challenge infection.
复制标题
用携带衣原体主要外膜蛋白抗原的重组腺病毒转染的树突状细胞（DC）可引发针对生殖道攻击感染的保护性免疫反应。
DOI：
10.1139/o10-011
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Weiming Zhao
中科院分区：
文献类型：
--
作者：
H. Lü;Hong Wang;Hongyang Zhao;Lei Zhao;Qiang Chen;Mei Qi;Juan Liu;Han Yu;Xiu;Xi Yang;Weiming Zhao
沙眼衣原体是一种专性细胞内细菌病原体，是世界范围内性传播疾病的主要原因。尽管人们采取了各种策略来促进保护性疫苗的开发，但迄今为止还没有产生理想的疫苗。本研究用携带C.沙眼衣原体血清型E主要外膜蛋白基因（Ad-MOMP），并研究其诱导特异性保护抵抗生殖道衣原体攻击感染的能力。结果显示，Ad-MOMP体外转染DC后，DC表达CD 80和MHC-II分子，分泌IL-12，刺激T细胞增殖。受刺激的T细胞分泌的IFN-γ水平也显著上调。当Ad-MOMP转染的DCs过继性静脉转移到幼稚小鼠时，它们产生Th 1偏向的细胞因子产生和对C.沙眼更重要的是，用Ad-MOMP-DC免疫的小鼠对生殖道攻击感染具有保护作用，表现为较低的体重减轻、较低的衣原体负荷和较不严重的病理变化。结论：Ad-MOMP转染的DC能诱导有效的抗C.沙眼生殖器感染
Chlamydia trachomatis, an obligate intracellular bacterial pathogen, is the major cause of sexually transmitted diseases worldwide. Although a variety of strategies have been taken to promote the development of a protective vaccine, no ideal vaccine has been generated so far. In this study, we transfected dendritic cells (DCs) with recombinant adenovirus carrying C. trachomatis serovar E major outer membrane protein gene (Ad-MOMP), and investigated their ability to induce specific protection against genital tract chlamydial challenge infection. The results showed that when DCs were transfected with Ad-MOMP in vitro, the DCs exhibited increased expression of CD80 and MHC-II molecules as well as enhanced IL-12 secretion and were able to stimulate T-cell proliferation. The level of IFN-gamma secreted by stimulated T cells was also up-regulated significantly. When the Ad-MOMP transfected DCs were adoptively transferred intravenously to naive mice, they generated Th1-biased cytokine production and mucosal IgA responses specific for C. trachomatis. More importantly, the mice immunized with Ad-MOMP-DC mounted protection against genital tract challenge infection, shown by lower body mass loss, lower chlamydial loads, and less severe pathological changes. In conclusion, Ad-MOMP transfected DCs are capable of inducing effective protective immune responses against C. trachomatis genital infection.
影响因子：
120.7
作者：
Parish, WL;Laumann, EO;Ng, KH
通讯作者：
Ng, KH