Membrane fluidity of tetramyristoyl cardiolipin (TMCL) liposomes studied by chronoamperometric monitoring of their adhesion and spreading at the surface of a mercury electrode

Membrane fluidity of tetramyristoyl cardiolipin (TMCL) liposomes studied by chronoamperometric monitoring of their adhesion and spreading at the surface of a mercury electrode
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通过计时电流法监测四肉豆蔻酰心磷脂 (TMCL) 脂质体在汞电极表面的粘附和扩散来研究其膜流动性

DOI:
10.1007/s10008-012-1758-8
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发表时间:
2012
影响因子:
2.5
通讯作者:
Lorenz Schild
Lorenz Schild
中科院分区:
工程技术4区
文献类型:
--
作者:
Stefan Zander;Michael Hermes;Fritz Scholz;Andreas Gröning;Christiane A. Helm;Doris Vollmer;Uwe Lendeckel;Lorenz Schild

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合成的心磷脂1 ',3 ' -二[1,2-二myristoyll -sn-glycero-3-phospho]-sn-glycerol的巨大单层脂质体在固定的汞电极上产生计时电流峰。这些信号是由于脂质体在疏水汞表面的粘附和扩散。电势依赖性表明,在零电荷点峰值频率最小,峰值频率在−0.2 V左右达到最大值,而峰值在−0.8 V时较小。脂质体的电化学行为表明了心磷脂的相变,这也可以用差示扫描量热法观察到。
Giant unilamellar liposomes of the synthetic cardiolipin 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol give chronoamperometric current peaks at a stationary mercury electrode. The signals are due to the adhesion and spreading of the liposomes on the hydrophobic mercury surface. The potential dependence shows a minimum of the peak frequency at the point of zero charge, a large maximum of peak frequency at about −0.2 V and a second, however, smaller maximum at −0.8 V. The electrochemical behaviour of the liposomes indicates phase transitions of the cardiolipin which could be also observed in differential scanning calorimetry.
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