Rickettsia actin-based motility occurs in distinct phases mediated by different actin nucleators.
Rickettsia actin-based motility occurs in distinct phases mediated by different actin nucleators.
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DOI：
10.1016/j.cub.2013.11.025
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发表时间：
2014-01-06
期刊：
影响因子：
9.2
通讯作者：
Welch, Matthew D.
中科院分区：
文献类型：
--
作者：
Reed, Shawna C. O.;Lamason, Rebecca L.;Risca, Viviana I.;Abernathy, Emma;Welch, Matthew D.
许多细胞内细菌病原体在感染期间经历基于肌动蛋白的运动以促进细胞-细胞传播。对于每种病原体，运动被认为是由一个单一的肌动蛋白聚合途径驱动。奇怪的是，斑点热群立克次体与其他病原体的不同之处在于具有两种肌动蛋白聚合蛋白。RickA是宿主Arp 2/3复合物的激活剂，最初被提出驱动运动。Sca 2是宿主formin的模拟物，后来被证明是运动所需的。它们的活动是否协调以及如何协调仍不清楚。在这里，我们表明，每种蛋白质在感染过程中的不同时间指导一个独立的模式的立克次体运动。入侵后早期，运动缓慢且蜿蜒，产生短而弯曲的肌动蛋白尾部，富含Arp 2/3复合物和cofilin。早期运动需要RickA和Arp 2/3复合物，并且与瞬时RickA定位于细菌极相关。在感染后期，运动更快，方向持久，导致长而直的肌动蛋白尾巴。晚期运动不依赖于Arp 2/3复合物和RickA，并且需要Sca 2，Sca 2在细菌极处积累。两种运动途径都促进细胞与细胞的传播。利用两个肌动蛋白组装途径的能力可能允许立克次体建立细胞内生态位，并在整个长期感染过程中在不同细胞之间传播。
Many intracellular bacterial pathogens undergo actin-based motility to promote cell-cell spread during infection. For each pathogen, motility was assumed to be driven by a single actin polymerization pathway. Curiously, spotted-fever-group Rickettsia differ from other pathogens in possessing two actin polymerizing proteins. RickA, an activator of the host Arp2/3 complex, was initially proposed to drive motility. Sca2, a mimic of host formins, was later shown to be required for motility. Whether and how their activities are coordinated has remained unclear. Here, we show that each protein directs an independent mode of Rickettsia parkeri motility at different times during infection. Early after invasion, motility is slow and meandering, generating short, curved actin tails that are enriched with Arp2/3 complex and cofilin. Early motility requires RickA and Arp2/3 complex, and is correlated with transient RickA localization to the bacterial pole. Later in infection, motility is faster and directionally persistent, resulting in long, straight actin tails. Late motility is independent of Arp2/3 complex and RickA, and requires Sca2, which accumulates at the bacterial pole. Both motility pathways facilitate cell-to-cell spread. The ability to exploit two actin assembly pathways may allow Rickettsia to establish an intracellular niche and spread between diverse cells throughout a prolonged infection.
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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