Leptin is involved in acrosome reaction by facilitating activation of MAPK cascades in the Chinese mitten crab, Eriocheir sinensis
Leptin is involved in acrosome reaction by facilitating activation of MAPK cascades in the Chinese mitten crab, Eriocheir sinensis
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瘦素通过促进中华绒螯蟹 MAPK 级联的激活参与顶体反应
DOI：
10.1163/15707563-20191095
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发表时间：
2020
期刊：
影响因子：
1.2
通讯作者：
Wang Qun
中科院分区：
文献类型：
--
作者：
Li Qing;Zhao Haitao;He Lin;Yang Hongdan;Wang Qun
瘦素的作用已在多项研究中得到证实，包括啮齿类动物和人类生殖中细胞外信号调节激酶 (ERK1/2) 的激活苏氨酸磷酸化。我们前期的研究表明，丝裂原激活蛋白激酶（MAPK）级联反应ERK、P38和c-Jun氨基末端激酶（JNK）参与中华绒螯蟹的精子发生和顶体反应。因此，本研究的目的是探讨钙离子载体A23187诱导螃蟹精子顶体反应过程中瘦素及其受体（LepR）的表达，以及瘦素对MAPK级联反应的影响。成功的蛋白质印迹显示，在测试的男性生殖组织中，瘦素分别有 16 kDa 条带，肥胖受体 (LepR) 分别有 120 kDa 和 90 kDa 条带。瘦素和 LepR 均定位于精子细胞的前顶体囊泡和顶帽 (AC)，表明它们在随后的顶体反应中发挥作用。此外，瘦素可以增强顶体反应，而抗LepR抗体会减弱这种作用。随后，我们研究了瘦素对 MAPK 级联的影响。结果表明，在蟹精子顶体反应过程中，瘦素主要激活顶帽中ERK、P38和JNK蛋白的磷酸化。这项研究探讨了瘦素对精子的作用，并表明瘦素可能在顶体反应过程中诱导与精子相关的分子变化。
The role of leptin has been documented in several studies, including activated threonine phosphorylation of extracellular signal-regulated kinase (ERK1/2) in the reproduction of rodents and humans. Our previous studies have demonstrated that mitogen-activated protein kinase (MAPK) cascades ERK, P38, and c-Jun N-terminal kinase (JNK) are involved in the spermatogenesis and acrosome reaction of Eriocheir sinensis. Therefore, the aim of this study was to investigate the expression of leptin and its receptor (LepR), and the effect of leptin on MAPK cascades during calcium ionophore A23187-induced spermatozoa acrosome reaction in crabs. Successful western blotting revealed a 16 kDa band for leptin, and 120 kDa and 90 kDa bands for the obese receptor (LepR), respectively, in the tested male reproductive tissues. Both leptin and LepR were localized at the pro-acrosomal vesicle and apical cap (AC) of spermatids, suggesting their role in the subsequent acrosome reaction. Moreover, acrosome reaction can be enhanced by leptin, and this effect decreased due to the anti-LepR antibody. Afterwards, we investigated the effects of leptin on MAPK cascades. The results showed that leptin mainly activated the phosphorylation of ERK, P38 and JNK proteins in the apical cap during the acrosome reaction in crab spermatozoa. This study addresses the role of leptin on spermatozoa, and suggests that leptin may induce molecular changes associated with spermatozoa during acrosome reaction.
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影响因子：
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作者：
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作者：
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通讯作者：
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影响因子：
64.8
作者：
ZHANG, YY;PROENCA, R;FRIEDMAN, JM
通讯作者：
FRIEDMAN, JM