Compositional assessment of bone by Raman spectroscopy.
Compositional assessment of bone by Raman spectroscopy.
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DOI：
10.1039/d1an01560e
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发表时间：
2021-12-06
期刊：
影响因子：
--
通讯作者：
Nyman JS
中科院分区：
文献类型：
--
作者：
Unal M;Ahmed R;Mahadevan-Jansen A;Nyman JS
拉曼光谱（RS）用于分析骨的理化性质，因为它是非破坏性的，需要最少的样品制备。通过二十多年的研究，涉及通过RS测量骨基质的矿物质-基质比、B型碳酸盐取代、结晶度和其他组成特征，存在多种方法来从骨获取拉曼信号，处理这些信号，并确定峰比，包括亚峰比以及最显著的拉曼峰的半峰全宽，nu 1磷酸盐（ν 1 PO 4）。选择使用哪些方法并不总是很清楚。在这里，我们描述了RS仪器的组成部分，以及它们如何影响从骨采集的拉曼光谱的质量，因为采集的信噪比和伴随的背景荧光决定了拉曼光谱的预处理。我们还描述了常见的方法和挑战，在准备收购的光谱测定骨的基质特性。本文还通过示例说明预处理拉曼信号和确定骨成分特性的方法如何影响RS对实验组之间潜在差异的敏感性，为RS分析骨骼提供指导。还注意到用于确定酰胺I条带的亚峰比率的去卷积方法，作为评估I型胶原蛋白特征的一种方式。我们就RS在骨中的应用提供了建议和意见，目的是提高研究的重现性，并巩固RS作为骨研究领域的一项有价值的技术。
Raman spectroscopy (RS) is used to analyze the physiochemical properties of bone because it is non-destructive and requires minimal sample preparation. With over two decades of research involving measurements of mineral-to-matrix ratio, Type-B carbonate substitution, crystallinity, and other compositional characteristics of the bone matrix by RS, there are multiple methods to acquire Raman signals from bone, to process those signals, and to determine peak ratios including sub-peak ratios as well as the full-width at half maximum of the most prominent Raman peak, which is nu1 phosphate (ν1PO4). Selecting which methods to use is not always clear. Herein, we describe the components of RS instruments and how they influence the quality of Raman spectra acquired from bone because signal-to-noise of the acquisition and the accompanying background fluorescence dictate the pre-processing of the Raman spectra. We also describe common methods and challenges in preparing acquired spectra for the determination of matrix properties of bone. This article also serves to provide guidance for the analysis of bone by RS with examples of how methods for pre-processing the Raman signals and for determining properties of bone composition affect RS sensitivity to potential differences between experimental groups. Attention is also given to deconvolution methods that are used to ascertain sub-peak ratios of the amide I band as a way to assess characteristics of collagen type I. We provide suggestions and recommendations on the application of RS to bone with the goal of improving reproducibility across studies and solidify RS as a valuable technique in the field of bone research.
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DOI：
10.1002/jbmr.2759
发表时间：
2016-05
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Boskey AL;Donnelly E;Boskey E;Spevak L;Ma Y;Zhang W;Lappe J;Recker RR
通讯作者：
Recker RR
影响因子：
2.4
作者：
Bi, Xiaohong;Patil, Chetan A.;Nyman, Jeffry S.
通讯作者：
Nyman, Jeffry S.
影响因子：
3.5
作者：
Chen, TC;Shea, DA;Morris, MD
通讯作者：
Morris, MD
影响因子：
2.8
作者：
Chen K;Massie C;Berger AJ
通讯作者：
Berger AJ
影响因子：
4.2
作者：
Creecy A;Uppuganti S;Merkel AR;O'Neal D;Makowski AJ;Granke M;Voziyan P;Nyman JS
通讯作者：
Nyman JS