Regional distribution of maternal messenger RNA in the amphibian oocyte
Regional distribution of maternal messenger RNA in the amphibian oocyte
复制标题
两栖动物卵母细胞中母体信使RNA的区域分布
DOI：
10.1016/0012-1606(85)90134-4
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发表时间：
1985
影响因子：
2.7
通讯作者：
E. Barklis
中科院分区：
文献类型：
--
作者：
M. L. King;E. Barklis
我们发现，在Xenopus卵母细胞的动植物轴上，以mRNAs形式存在的信息是非随机分布的。从该轴不同区域分离的多腺苷化RNA在无细胞体系中翻译，产物经双向凝胶电泳法分离。使用计算机程序对产生的蛋白质产物进行比较后发现，特定的可翻译的mRNAs在受精前定位于卵母细胞内。17个mRNAs在其亚细胞位置上表现出显著的质或量差异。这些序列占检测到的全部可翻译多聚腺苷酸化RNA的4%。Xenopus发育过程中这些信息的不对称分离可能与细胞命运的决定有关。
We show that there is a non-random distribution of information in the form of mRNAs along the animal/vegetal axis ofXenopusoocytes. Polyadenylated RNA isolated from different regions along this axis was translated in a cell-free system, and the products were fractionated by two-dimensional gel electrophoresis. A comparison of the resulting protein products using a computer program has established that specific translatable mRNAs are regionally localized within the oocyte prior to fertilization. Seventeen mRNAs show significant qualitative or quantitative differences in their subcellular location. These sequences comprise as much as 4% of the total detected translatable polyadenylated RNA. Asymmetric segregation of these messages duringXenopusdevelopment may be involved in the determination of cell fate.
影响因子：
2.7
作者：
JEFFERY, WR;TOMLINSON, CR;BRODEUR, RD
通讯作者：
BRODEUR, RD
两栖类卵母细胞中散布的聚腺苷酸RNA (poly(A) RNA) 不可翻译。
影响因子：
5.6
作者：
Richter,JD;Smith,LD;Anderson,DM;Davidson,EH
通讯作者：
Davidson,EH
二维凝胶放射自显影计算机分析的简化方法。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Garrison,JC;Johnson,ML
通讯作者：
Johnson,ML