Expression of cyclo-oxygenase types-1 and -2 in human myometrium throughout pregnancy.
Expression of cyclo-oxygenase types-1 and -2 in human myometrium throughout pregnancy.
复制标题
整个妊娠期间人类子宫肌层中环加氧酶 1 型和 -2 型的表达。
DOI：
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发表时间：
1999
影响因子：
4
通讯作者：
Phillip R. Bennett
中科院分区：
文献类型：
--
作者：
Donna M. Slater;W. Dennes;J. S. Campa;Lucilla Poston;Phillip R. Bennett
人类劳动与子宫内前列腺素合成增加有关。本研究的目的是检查两种异构体的中央前列腺素合成酶，环氧合酶（考克斯-1和考克斯-2）的表达在整个妊娠期的人子宫肌层和测试的假设，即考克斯在子宫肌层可能发挥作用，在劳动力发动。采用逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法分析考克斯-1和考克斯-2在mRNA水平的表达。考克斯-1 RNA或蛋白表达随胎龄或分娩均无显著变化。考克斯-2 mRNA和蛋白表达在足月时均升高，在临产前表达显著上调（P < 0.005）。这些数据表明，在足月子宫肌层中，考克斯-2而不是考克斯-1的上调介导了前列腺素合成的增加。观察到考克斯-2表达的增加先于分娩开始，表明考克斯-2在分娩开始中起作用，而不是其存在仅仅是分娩的结果。
Human labour is associated with increased prostaglandin synthesis within the uterus. The aim of this study was to examine the expression of the two isoforms of the central prostaglandin synthetic enzyme, cyclo-oxygenase (COX-1 and COX-2) in human myometrium throughout pregnancy and to test the hypothesis that COX in the myometrium may play a role in labour onset. Expression of COX-1 and COX-2 at the mRNA level was analysed using reverse transcriptase-polymerase chain reaction (RT-PCR) and at the protein level using Western blotting. No significant changes of COX-1 RNA or protein expression were observed either with gestational age or labour. COX-2 mRNA and protein expression increased at term with significant up-regulation occurring prior to the onset of labour (P < 0.005). These data would suggest that up-regulation of COX-2, rather than COX-1, mediates increased prostaglandin synthesis in human myometrium at term. The increased COX-2 expression observed preceded labour onset, suggesting that COX-2 has a role in labour onset, rather than its presence merely a consequence of labour.
DOI：
10.1210/jcem.79.3.8077379
发表时间：
1994-09
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
J. Zuo;Z M Lei;C V Rao;M. Pietrantoni;V D Cook
通讯作者：
J. Zuo;Z M Lei;C V Rao;M. Pietrantoni;V D Cook