Efficacy of adenovirally expressed soluble TRAIL in human glioma organotypic slice culture and glioma xenografts.
Efficacy of adenovirally expressed soluble TRAIL in human glioma organotypic slice culture and glioma xenografts.
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DOI：
10.1038/cddis.2010.95
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发表时间：
2011-02-24
影响因子：
9
通讯作者：
中科院分区：
文献类型：
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Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in malignant cells, including gliomas, and is currently in anticancer clinical trials. However, the full-length and tagged forms of TRAIL, unlike the untagged ligand (soluble TRAIL (sTRAIL)), exhibits toxicity against normal cells. Here, we report the generation and testing of an adenovirus (AdsTRAIL) that expresses untagged sTRAIL in an intracranial xenograft model and a human glioma organotypic slice culture model. AdsTRAIL efficiently induced apoptosis in glioma cell lines, including those resistant to sTRAIL, but not in normal human astrocytes (NHAs). It inhibited anchorage-independent glioma growth and exerted a bystander effect in transwell assays. Intratumoral injections of AdsTRAIL in a rodent intracranial glioma model resulted in reduced tumor growth and improved survival compared with Ad-enhanced green fluorescent protein (EGFP)- or vehicle-treated controls without toxicity. Human glioma organotypic slices treated with AdsTRAIL demonstrated apoptosis induction and caspase activation.
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影响因子：
3.7
作者：
Jeong, Moonsup;Kwon, Yong-Sam;Park, Soon-Hye;Kim, Chae-Young;Jeun, Sin-Soo;Song, Kang-Won;Ko, Yong;Robbins, Paul D.;Billiar, Timothy R.;Kim, Byong-Moon;Seol, Dai-Wu
通讯作者：
Seol, Dai-Wu
影响因子：
56.9
作者：
Pan, GH;ORourke, K;Dixit, VM
通讯作者：
Dixit, VM
影响因子：
168.9
作者：
Nitsch, R;Bechmann, I;Zipp, F
通讯作者：
Zipp, F
影响因子：
4.5
作者：
Kim KU;Seo SY;Heo KY;Yoo YH;Kim HJ;Lee HS;Choi SS;Hwang TH;Lee HJ
通讯作者：
Lee HJ
影响因子：
9.2
作者：
Marsters, SA;Pitti, RM;Ashkenazi, A
通讯作者：
Ashkenazi, A