Insights into the genomic evolution and the alkali tolerance mechanisms of Agaricus sinodeliciosus by comparative genomic and transcriptomic analyses.
Insights into the genomic evolution and the alkali tolerance mechanisms of Agaricus sinodeliciosus by comparative genomic and transcriptomic analyses.
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DOI：
10.1099/mgen.0.000928
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发表时间：
2023-03
影响因子：
3.9
通讯作者：
Zhao, Rui-Lin
中科院分区：
文献类型：
--
作者：
Ling, Zhi-Lin;Cao, Bin;Hu, Song-Nian;Geng, Jia-Ning;Liu, Fei;Liu, Dong-Mei;Zhao, Rui-Lin
中华蘑菇是我国西北地区珍稀野生食用菌，自然生长在轻度盐碱土壤中，这在蘑菇中也是不常见的。A. sinodeliciosus是一种很有潜力的模式生物，可以用来解释蘑菇的耐盐碱机制和揭示相关的生理过程。在这里，我们提供了一个高质量的基因组A。sinodeliciosus。比较基因组分析显示A.中华绒螯蟹在盐碱环境下经历了一段孤独的进化史，其基因组结构发生了许多变化，如基因家族收缩、反转录转座子扩增和适应基因的快速进化等。耐盐碱试验表明，微碱性对该菌菌丝生长和子实体形成有一定的影响。转录组学分析表明，这些基因与A.中华松藻在弱碱性条件下能被激活。特别是"淀粉和蔗糖代谢“、”氨基酸生物合成“和”苯丙素类生物合成“途径对A. sinodeliciosus。与植物和丛枝菌根真菌一样，在腐烂真菌A.在微碱性条件下，细胞内小分子物质的生物合成可以增强，以对抗由微碱性引起的渗透胁迫和氧化胁迫;而在微碱性条件下，细胞壁渗透性增加，单萜醇的生物合成受到抑制。本研究为深入了解A.中华绒螯蟹对盐碱环境的耐受性。助理中华蘑菇基因组是研究蘑菇属进化和生态学的重要资源。
Agaricus sinodeliciosus is a rare wild edible mushroom from northwest China, and grows naturally in mild saline-alkali soil, which is also unusual in mushrooms. A. sinodeliciosus represents a potential model organism for explaining saline-alkali tolerance mechanisms and revealing related physiological processes in mushrooms. Here, we provide a high-quality genome of A. sinodeliciosus. Comparative genomic analyses reveal A. sinodeliciosus has numerous changes to its genome organization after a solitary evolutionary history under saline-alkali environments, such as gene family contraction, retrotransposon expansion and rapid evolution of adaptative genes. Our saline and alkali tolerance tests show that mycelium growth and fruit body formation of this species are effected by mild alkalinity. Transcriptomic analyses reveal that genes involved in carbon and nitrogen utilization, cell stability and fruit body formation of A. sinodeliciosus could be activated under mildly alkaline conditions. In particular, the ‘starch and sucrose metabolism’, ‘biosynthesis of amino acids’ and ‘phenylpropanoid biosynthesis’ pathways are important for mildly alkaline tolerance of A. sinodeliciosus. Like plants and arbuscular mycorrhizal fungi, in the rot fungus A. sinodeliciosus, the biosynthesis of intracellular small molecules could be enhanced to counter osmotic and oxidative stresses caused by mild alkalinity, and the biosynthesis of monolignol could be suppressed to increase cell wall infiltrates under mildly alkaline conditions. This research provides an understanding of the genomic evolution and mechanisms of A. sinodeliciosus in tolerance to saline-alkali environments. The A. sinodeliciosus genome constitutes a valuable resource for evolutionary and ecological studies of Agaricus.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
14.9
作者：
Dimmer EC;Huntley RP;Alam-Faruque Y;Sawford T;O'Donovan C;Martin MJ;Bely B;Browne P;Mun Chan W;Eberhardt R;Gardner M;Laiho K;Legge D;Magrane M;Pichler K;Poggioli D;Sehra H;Auchincloss A;Axelsen K;Blatter MC;Boutet E;Braconi-Quintaje S;Breuza L;Bridge A;Coudert E;Estreicher A;Famiglietti L;Ferro-Rojas S;Feuermann M;Gos A;Gruaz-Gumowski N;Hinz U;Hulo C;James J;Jimenez S;Jungo F;Keller G;Lemercier P;Lieberherr D;Masson P;Moinat M;Pedruzzi I;Poux S;Rivoire C;Roechert B;Schneider M;Stutz A;Sundaram S;Tognolli M;Bougueleret L;Argoud-Puy G;Cusin I;Duek-Roggli P;Xenarios I;Apweiler R
通讯作者：
Apweiler R
影响因子：
10.7
作者：
Darriba, Diego;Posada, David;Flouri, Tomas
通讯作者：
Flouri, Tomas
影响因子：
8.1
作者：
Falguera, Victor;Pagan, Jordi;Ibarz, Albert
通讯作者：
Ibarz, Albert
影响因子：
3.7
作者：
English AC;Richards S;Han Y;Wang M;Vee V;Qu J;Qin X;Muzny DM;Reid JG;Worley KC;Gibbs RA
通讯作者：
Gibbs RA