A histochemical study on lectin binding in the immature enamel and secretory ameloblasts of rat incisors.
A histochemical study on lectin binding in the immature enamel and secretory ameloblasts of rat incisors.
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大鼠门牙未成熟牙釉质和分泌成釉细胞中凝集素结合的组织化学研究。
DOI：
10.1620/tjem.155.139
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
M. Kagayama
中科院分区：
文献类型：
--
作者：
H. Akita;Y. Kobayashi;M. Kagayama
四种凝集素-荧光标记物缀合物（Con A-F、MPA-F、PNA-F、WGA-R）用于通过荧光显微镜观察它们在组织切片上的结合位点。在石蜡包埋组织切片上，用MPA-F和WGA-R对未成熟釉质、Tomes'突起和成釉细胞远端胞质进行染色。MPA-和WGA-结合糖缀合物似乎是有机釉质基质的主要成分，因为从组织切片的强烈荧光。PNA-F在成釉细胞远侧胞质中央部分有阳性染色，未成熟釉质和Tomes突无阳性染色。这表明PNA结合的糖复合物是修饰其寡糖链过程中的中间体或高尔基区的元件。成釉细胞的Tomes突起、胞浆远端及核周围用ConA-F染色。Con A结合的糖缀合物除了那些定位于Tomes'过程之外，也可能是中间体和/或细胞内元件，如PNA结合的糖缀合物。中间层细胞用ConA-F、MPA-F和WGA-R染色。用ConA-F和WGA-R对星网状细胞和外釉上皮细胞进行染色。结果表明，釉质蛋白，未成熟釉质的主要有机成分，是高度可能的候选人MPA和WGA结合的糖缀合物。
Four lectin-fluorescent marker conjugates (Con A-F, MPA-F, PNA-F, WGA-R) were used for visualizing their binding sites on tissue sections by fluorescent microscopy. The immature enamel, Tomes' processes and distal cytoplasm of ameloblasts were stained with MPA-F and WGA-R on paraformaldehyde-fixed and paraffin-embedded tissue sections. The MPA- and WGA-binding glycoconjugates seemed to be main components of the organic enamel matrix because of the intense fluorescence from the tissue sections. The central portion of distal cytoplasm of ameloblasts was stained with PNA-F but the immature enamel and Tomes' processes were not. This suggests the PNA-binding glycoconjugates to be intermediates during the process of modifying their oligosaccharide chains or to be elements of Golgi area. Tomes' processes, the distal cytoplasm and periphery of nuclei of ameloblasts were stained with Con A-F. The Con A-binding glycoconjugates except those localizing on Tomes' processes may also be intermediates and/or intracellular elements like the PNA-binding glycoconjugates. Cells of stratum intermedium were stained with Con A-F, MPA-F and WGA-R. Cells of stellate reticulum and outer enamel epithelium were stained with Con A-F and WGA-R. It was indicated that enamel proteins, major organic components of immature enamel, are highly possible candidates for the MPA- and WGA-binding glycoconjugates.
发育中的牛牙釉质的有机基质与钙结合。
影响因子：
3
作者：
Drinkard,C;Gibson,L;Crenshaw,MA;Bawden,JW
通讯作者：
Bawden,JW
DOI：
10.1016/0006-291x(85)91964-3
发表时间：
1985-01-01
影响因子：
3.1
作者：
SNEAD, ML;LAU, EC;SLAVKIN, HC
通讯作者：
SLAVKIN, HC