In Vivo Sensing of pH in Tomato Plants Using a Low-Cost and Open-Source Device for Precision Agriculture.
In Vivo Sensing of pH in Tomato Plants Using a Low-Cost and Open-Source Device for Precision Agriculture.
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DOI：
10.3390/bios12070447
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发表时间：
2022-06-23
期刊：
影响因子：
5.4
通讯作者：
Haseloff, Jim
中科院分区：
文献类型：
--
作者：
Ruiz-Gonzalez, Antonio;Kempson, Harriet;Haseloff, Jim
精准农业传感设备的开发对于提高作物产量和限制由于人口增长而导致的粮食生产短缺至关重要。然而，目前的方法不能提供关于植物生理状态的直接信息，降低了传感的准确性。植入式植物监测装置的开发代表了这一领域的进步，使直接评估植物中的关键生物标志物成为可能。然而，现有的设备价格昂贵，不能用于长期应用。本文介绍了氧化钌基纳米膜在植物体内pH监测中的应用。该传感器采用低成本电沉积的方法制备，最终装置可以成功地用于监测木质部汁液pH值至少10小时。由于RuO2纳米颗粒具有生物相容性和化学稳定性，因此选择RuO2纳米颗粒作为传感材料。为了降低传感器的噪声率和漂移，采用气溶胶法（>GBP 50）沉积了一层由纤维素/PDMS混合材料组成的保护层，其中包括现成的设备，可以很好地控制膜厚。制备了厚度为80 nm，粗糙度小于3 nm的纳米薄膜。该薄膜使漂移降低了7倍，同时保留了传感器对H+离子的选择性。传感装置通过植入番茄植株进行了体内测试。此外，还使用低成本的Wio终端设备监测湿度和温度等环境参数，并将数据无线发送到在线服务器。最后研究了植物组织与基于金属氧化物的传感器之间的相互作用，证明在传感膜和木质部之间形成了木质化层。因此，本工作首次报道了一种低成本的电化学传感器，可用于连续监测木质部汁液中的pH值。该装置可以很容易地进行修改，以提高植入植物组织后的长期性能，代表了精准农业技术发展的一步。
The development of sensing devices for precision agriculture is crucial to boost crop yields and limit shortages in food productions due to the growing population. However, current approaches cannot provide direct information about the physiological status of the plants, reducing sensing accuracy. The development of implanted devices for plant monitoring represents a step forward in this field, enabling the direct assessment of key biomarkers in plants. However, available devices are expensive and cannot be used for long-term applications. The current work presents the application of ruthenium oxide-based nanofilms for the in vivo monitoring of pH in plants. The sensors were manufactured using the low-cost electrodeposition of RuO2 films, and the final device could be successfully incorporated for the monitoring of xylem sap pH for at least 10 h. RuO2 nanoparticles were chosen as the sensing material due to its biocompatibility and chemical stability. To reduce the noise rates and drift of the sensors, a protective layer consisting of a cellulose/PDMS hybrid material was deposited by an aerosol method (>GBP 50), involving off-the-shelf devices, leading to a good control of film thickness. Nanometrically thin films with a thickness of 80 nm and roughness below 3 nm were fabricated. This film led to a seven-fold decrease in drift while preserving the selectivity of the sensors towards H+ ions. The sensing devices were tested in vivo by implantation inside a tomato plant. Environmental parameters such as humidity and temperature were additionally monitored using a low-cost Wio Terminal device, and the data were sent wirelessly to an online server. The interactions between plant tissues and metal oxide-based sensors were finally studied, evidencing the formation of a lignified layer between the sensing film and xylem. Thus, this work reports for the first time a low-cost electrochemical sensor that can be used for the continuous monitoring of pH in xylem sap. This device can be easily modified to improve the long-term performance when implanted inside plant tissues, representing a step forward in the development of precision agriculture technologies.
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影响因子：
5.8
作者：
Diacci C;Abedi T;Lee JW;Gabrielsson EO;Berggren M;Simon DT;Niittylä T;Stavrinidou E
通讯作者：
Stavrinidou E
DOI：
10.1088/0960-1317/23/3/035007
发表时间：
2013-03-01
影响因子：
2.3
作者：
Lee, Jihoon;Kim, Jungwoo;Cho, Hyoung J.
通讯作者：
Cho, Hyoung J.
影响因子：
4.3
作者：
LEE, YS;SATTER, RL
通讯作者：
SATTER, RL
DOI：
10.3390/s90604955
发表时间：
2009
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Kurzweil P
通讯作者：
Kurzweil P
DOI：
10.3390/nano11040938
发表时间：
2021-04-07
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
--
作者：
Ruiz-Gonzalez A;Choy KL
通讯作者：
Choy KL