Epistatic association mapping in homozygous crop cultivars.
Epistatic association mapping in homozygous crop cultivars.
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纯合作物品种的上位关联作图
DOI：
10.1371/journal.pone.0017773
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发表时间：
2011-03-15
期刊：
影响因子：
3.7
通讯作者：
Zhang YM
中科院分区：
文献类型：
--
作者：
Lü HY;Liu XF;Wei SP;Zhang YM
复杂性状的遗传分析在作物育种中起着至关重要的作用。然而，迄今为止，遗传分析和作物育种是分开进行的。在这项研究中，我们设计了一种新的方法，整合上位性关联分析在作物品种的育种设计。首先，我们提出了一个上位关联作图（EAM）的方法在纯合作物品种。利用复杂性状的表型值和沿着的分子标记信息进行EAM。在我们的EAM中，所有的主效应数量性状位点（QTL），环境效应，QTL与环境的互作和QTL与QTL的互作被纳入一个完整的模型，并估计经验贝叶斯方法。一系列的Monte Carlo模拟进行了确认的新方法的可靠性。然后利用检测到的QTL信息挖掘每个位点的新等位基因，设计优良杂交组合。最后，采用分层随机抽样的方法，对我国6个地理生态型的215个大豆品种进行了粒长遗传基础的剖析。结果发现了19个主效QTL和3个上位性QTL，挖掘了10多个新等位基因，并预测了大青豆和郑州790034等3个优良亲本组合。
The genetic dissection of complex traits plays a crucial role in crop breeding. However, genetic analysis and crop breeding have heretofore been performed separately. In this study, we designed a new approach that integrates epistatic association analysis in crop cultivars with breeding by design. First, we proposed an epistatic association mapping (EAM) approach in homozygous crop cultivars. The phenotypic values of complex traits, along with molecular marker information, were used to perform EAM. In our EAM, all the main-effect quantitative trait loci (QTLs), environmental effects, QTL-by-environment interactions and QTL-by-QTL interactions were included in a full model and estimated by empirical Bayes approach. A series of Monte Carlo simulations was performed to confirm the reliability of the new method. Next, the information from all detected QTLs was used to mine novel alleles for each locus and to design elite cross combination. Finally, the new approach was adopted to dissect the genetic basis of seed length in 215 soybean cultivars obtained, by stratified random sampling, from 6 geographic ecotypes in China. As a result, 19 main-effect QTLs and 3 epistatic QTLs were identified, more than 10 novel alleles were mined and 3 elite parental combinations, such as Daqingdou and Zhengzhou790034, were predicted.
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