Comparison of bacterial communities in soil samples with and without tomato bacterial wilt caused by Ralstonia solanacearum species complex
Comparison of bacterial communities in soil samples with and without tomato bacterial wilt caused by Ralstonia solanacearum species complex
复制标题
有和没有由青枯菌复合体引起番茄青枯病的土壤样品中细菌群落的比较
DOI：
10.1186/s12866-020-01774-y
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发表时间：
2020-01
期刊：
影响因子：
4.2
通讯作者：
Li Peng
中科院分区：
文献类型：
--
作者：
Zhang Ying;Hu Anna;Zhou Jianuan;Zhang Wenfei;Li Peng
背景
青枯雷尔氏菌（Ralstonia solanacearum）是最臭名昭著的土传植物病原菌之一。它会导致严重的枯萎病，对许多重要的经济作物造成致命影响。抑病土壤中的微生物对作物的抗病性有重要作用，因此，评价抑病土壤（SS）和良病土壤（CS）中微生物群落及其相互作用特征将有助于了解作物的抗病机制。为此，分析了土壤细菌群落结构、与土壤化学性质的相关性、SS（近3年未发病）和CS（发生严重青枯病）的相互作用网络。
结果
SS的细菌群落多样性指数较高，类诺卡氏菌属、盖氏菌属和Anaerolineaceae的相对丰度显著高于CS。此外，主要属芽孢杆菌属、norank_o_Gaiellales、Roseiflexus和norank_o_gemmatimonadaceae的相对丰度显著高于CS。在属水平上的回归分析表明，有效磷在细菌群落分布中起着关键作用，其作用与土壤pH、有机质含量、碱解氮和速效钾含量呈负相关。互作网络分析进一步表明，SS网络在属水平上存在较大的多样性，并形成稳定的网络。此外，分枝杆菌、蓝细菌和红细菌是维持网络稳定性的关键组成部分。7组矫形器组在SS和CS之间表现出显着差异。此外，还筛选出了55株具有明显拮抗活性的细菌。从健康番茄植株根际土壤中分离鉴定了番茄青枯菌。
结论
研究结果表明，CS和SS样品的细菌多样性和相互作用网络存在差异，为青枯病的研究提供了良好的基础。
Background
Ralstonia solanacearum is one of the most notorious soil-borne phytopathogens. It causes a severe wilt disease with deadly effects on many economically important crops. The microbita of disease-suppressive soils are thought that they can contribute to the disease resistance of crop plants, thus, evaluation of the microbial community and their interaction characteristics between suppressive soil (SS) and conducive soil (CS) will help to understand resistance mechanism. To do this, the bacterial community structure, correlation analysis with soil chemical properties, interaction network of SS (nearly no disease in three years), and CS (suffered heavy bacterial wilt disease) were analyzed.
Results
A higher bacterial community diversity index was found in SS, the relative abundance of Nocardioides, Gaiella and norank_f_Anaerolineaceae were significantly more than that of the CS. Moreover, the relative abundance of main genera Bacillus, norank_o_Gaiellales, Roseiflexus, and norank_o_Gemmatimonadaceae were significantly more than that of the CS. Redundancy analysis at the genus level indicated that the available phosphate played a key role in the bacterial community distribution, and its role was negatively correlated with soil pH, organic matter content, alkali-hydrolyzable nitrogen, and available potassium contents. Interaction network analysis further demonstrated that greater diversity at the genus level existed in the SS network and formed a stable network. Additionally, the species of Mycobacterium, Cyanobacteria, and Rhodobiaceae are the key components that sustain the network stability. Seven clusters of orthologous groups exhibited significant differences between SS and CS. Moreover, 55 bacterial strains with distinct antagonistic activities to R. solancearum were isolated and identified from the healthy tomato plant rhizosphere soil of the CS.
Conclusions
Our findings indicate that the bacterial diversity and interaction network differed between the CS and SS samples, providing a good foundation in the study of bacterial wilt.
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影响因子：
5.3
作者：
通讯作者：
--
影响因子：
4.6
作者：
Wang R;Zhang H;Sun L;Qi G;Chen S;Zhao X
通讯作者：
Zhao X
系统型I菌株EP1的基因组分析揭示了与Ralstonia solanacearum物种复合物中其他菌株的显着差异。
影响因子：
5.2
作者：
Li P;Wang D;Yan J;Zhou J;Deng Y;Jiang Z;Cao B;He Z;Zhang L
通讯作者：
Zhang L
影响因子：
1.5
作者：
Tao Tian;Xiaogang Wu;Huiming Duan;Li-qun Zhang
通讯作者：
Tao Tian;Xiaogang Wu;Huiming Duan;Li-qun Zhang
影响因子：
4.5
作者：
Rosenzweig, Noah;Tiedje, James M.;Hao, Jianjun J.
通讯作者：
Hao, Jianjun J.