Effect of Direct-Current Electric Field on Enzymatic Activity and the Concentration of Laccase
Effect of Direct-Current Electric Field on Enzymatic Activity and the Concentration of Laccase
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直流电场对酶活性和漆酶浓度的影响
DOI：
10.1007/s12088-015-0523-y
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发表时间：
2015-03
影响因子：
3
通讯作者：
Feng, Tao
中科院分区：
文献类型：
--
作者：
Ren, Dajun;Li, Qian;Zhang, Shuqin;Feng, Tao
研究了直流电场对杂色曲霉漆酶的胞外酶活性、浓度等实验参数的影响。结果表明，漆酶对石墨电极末端pH的变化有显著影响。此外，它还增加了水的电导率。在本实验中，漆酶活力的最适pH和催化pH范围分别为3.0和2.5~4.0。6V的直流电压对漆酶活力有显著影响。漆酶活性在阳极区增强，但在阴极区受到强烈抑制。在电场作用下，漆酶的电荷特性发生变化，部分漆酶分子向阳极移动，产生轻微的迁移现象。本研究为漆酶与电学技术的结合奠定了基础，同时也为提高漆酶活力提供了新的方向。与固定化相比，利用电场进行固定化操作简单，不需要任何化学添加剂，具有很大的应用潜力。
This work investigates the effect of direct-current electric field on the extracellular enzymatic activity, concentration and other experimental parameters of laccase fromTrametes versicolor. The results showed that laccase could significantly contribute to the change of pH at the end of graphite electrode. In addition, it increased the electrical conductivity of the water. In the experiment, the optimum pH and catalytic pH range for laccase activity were 3.0 and pH 2.5–4.0. The application of 6 V direct current showed significant effects on the laccase enzyme activity. The activity of laccase was enhanced in the anodic region, but at the same time was strongly inhibited at the cathode. The electric charge characteristics of laccase were changed when exposed to electric field, and some laccases molecules moved to the anode, which produced a slight migration phenomenon. This study is the basis of combination of laccase and electrical technology, at the same time, providing a new direction of enhancing laccase activity. Compared to immobilization, using electric field is simple, no chemical additives, and great potential.
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影响因子：
3
作者：
F. Sheikhi;M. Roayaei Ardakani;N. Enayatizamir;S. Rodríguez-Couto
通讯作者：
F. Sheikhi;M. Roayaei Ardakani;N. Enayatizamir;S. Rodríguez-Couto
影响因子：
3
作者：
A. Yaropolov;Skorobogat'ko Ov;S. S. Vartanov-S.;S. Varfolomeyev
通讯作者：
A. Yaropolov;Skorobogat'ko Ov;S. S. Vartanov-S.;S. Varfolomeyev
DOI：
10.4236/abb.2010.13029
发表时间：
2010-08
期刊：
Advances in Bioscience and Biotechnology
影响因子：
--
作者：
I. Stoilova;A. Krastanov;V. Stanchev
通讯作者：
I. Stoilova;A. Krastanov;V. Stanchev
影响因子：
3.9
作者：
P. She;B. Song;X. Xing;M. Loosdrecht;Zheng Liu
通讯作者：
P. She;B. Song;X. Xing;M. Loosdrecht;Zheng Liu
影响因子：
5
作者：
Cassland, P;Jönsson, LJ
通讯作者：
Jönsson, LJ