High Expression of MUC5AC, MUC5B, and Layilin Plays an Essential Role in Prediction in the Development of Plastic Bronchitis Caused by MPP.
High Expression of MUC5AC, MUC5B, and Layilin Plays an Essential Role in Prediction in the Development of Plastic Bronchitis Caused by MPP.
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MUC5AC、MUC5B 和 Layilin 的高表达在预测 MPP 引起的塑料性支气管炎的发展中发挥重要作用
DOI：
10.3389/fmicb.2022.911228
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
塑料支气管炎(PB)是一种罕见的呼吸道疾病，可导致严重的呼吸道并发症，如呼吸衰竭和死亡。肺炎支原体感染是塑料支气管炎的主要病原学原因。然而，塑料支气管炎合并肺炎支原体肺炎(MPP)的发病机制尚未完全阐明。本文旨在探索早期预测MPP合并塑料支气管炎的生物标志物。我们利用蛋白质芯片来筛选健康、普通肺炎支原体肺炎(GMPP)和难治性肺炎支原体肺炎(RMPP)患者组中显著不同的蛋白质，在这些患者中，雷公藤红素显示出跨越生物信息技术的强大变化。接下来，我们证实了MUC5AC、MUC5B和laylin在MPP合并塑料支气管炎患者的支气管肺泡灌洗液(BALF)中高表达。进一步研究表明，Laylin水平与MUC5AC和MUC5B均呈正相关。通过受试者工作特征(ROC)分析，评价MUC5AC、MUC5B和LAYLIN对MPP合并PB的诊断价值。数据显示，这三项指标对MPP儿童塑料支气管炎的诊断能力相似。然后，我们使用不同浓度的社区获得性呼吸窘迫综合征(CADS)毒素或脂质相关膜蛋白(LAMP)来模拟体外实验。体外实验表明，CARDS毒素或LAMP可诱导A549细胞分泌MUC5AC、MUC5B、LAYLIN和促炎因子。提示MUC5AC、MUC5B和LAYLIN与MPP相关。MUC5AC、MUC5B和Laylin的高表达在预测MPP所致塑料支气管炎的发生发展中起重要作用。MUC5AC、MUC5B和laylin的高表达可能与疾病的严重程度有关。
Plastic bronchitis (PB) is a rare respiratory condition which can result in severe respiratory complications such as respiratory failure and death. Mycoplasma pneumoniae infection is a main etiology cause of plastic bronchitis. However, the pathogenesis of plastic bronchitis complicated by Mycoplasma pneumoniae pneumonia (MPP) has not yet been fully elucidated. Our article aims to explore biomarkers for early prediction of MPP cases complicated with plastic bronchitis. We utilized a protein chip to screen for significantly different proteins among the groups of healthy, general Mycoplasma pneumoniae pneumonia (GMPP) and refractory Mycoplasma pneumoniae pneumonia (RMPP) patients, where layilin exhibited a potent change across biology information technology. Next, we demonstrated the high expression of MUC5AC, MUC5B, and layilin in bronchoalveolar lavage fluid (BALF) of MPP cases complicated with plastic bronchitis. Further study suggested that the level of layilin had a positive correlation with both MUC5AC and MUC5B. A receiver operating characteristic (ROC) analysis was performed to assess the diagnostic values of MUC5AC, MUC5B, and layilin in MPP cases with PB. Data show that the three indicators have similar diagnostic ability for MPP children with plastic bronchitis. Then, we used different concentrations of community-acquired respiratory distress syndrome (CARDS) toxin or lipid-associated membrane proteins (LAMPs) to simulate an in vitro experiment. The in vitro assay revealed that CARDS toxin or LAMPs induced A549 cells to secrete MUC5AC, MUC5B, layilin, and proinflammatory factors. These findings suggest that MUC5AC, MUC5B, and layilin are correlated with MPP. The high expression of MUC5AC, MUC5B, and layilin play an essential role in prediction in the development of plastic bronchitis caused by MPP. The high expression of MUC5AC, MUC5B, and layilin may be relevant to the severity of illness.
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影响因子：
5.2
作者：
Su X;You X;Luo H;Liang K;Chen L;Tian W;Ye Z;He J
通讯作者：
He J
影响因子：
6.4
作者：
Caramori, Gaetano;Casolari, Paolo;Papi, Alberto
通讯作者：
Papi, Alberto
DOI：
10.1016/j.bbrc.2014.08.053
发表时间：
2014-09-12
影响因子：
3.1
作者：
Asano, Kota;Arito, Mitsumi;Kato, Tomohiro
通讯作者：
Kato, Tomohiro
影响因子：
4.1
作者：
Efrati, Ori;Sadeh-Gornik, Udi;Yahav, Yaacov
通讯作者：
Yahav, Yaacov
影响因子：
16.6
作者：
Hancock, Laura A.;Hennessy, Corinne E.;Schwartz, David A.
通讯作者：
Schwartz, David A.