Comparative transcriptional profiling of canine acanthomatous ameloblastoma and homology with human ameloblastoma.
Comparative transcriptional profiling of canine acanthomatous ameloblastoma and homology with human ameloblastoma.
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DOI：
10.1038/s41598-021-97430-0
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发表时间：
2021-09-07
影响因子：
4.6
通讯作者：
Grenier JK
中科院分区：
文献类型：
--
作者：
Peralta S;Duhamel GE;Katt WP;Heikinheimo K;Miller AD;Ahmed F;McCleary-Wheeler AL;Grenier JK
成釉细胞瘤是一种在人类中罕见的牙源性肿瘤，但在狗中发病率相对较高。由于犬棘瘤性成釉细胞瘤（CAA）与人类成釉细胞瘤（AM）具有共同的临床病理和分子特征，因此自发性CAA可以作为一种有用的疾病转化模型。然而，CAA的分子基础及其与AM的比较尚不完全清楚。在这项研究中，我们比较了CAA和AM的全球基因组表达谱，并通过使用大量RNA-seq方法评估了其牙齿起源。在这些研究中，健康的牙龈和犬口腔鳞状细胞癌作为对照。我们发现异常的RAS信号和上皮-间质转化细胞程序的激活参与了CAA的发病机制，并且CAA富含AM中上调的基因，包括在牙齿发育早期表达的基因，这表明它们具有高度的分子同源性。这些结果支持了一种模型，即自发性CAA家养狗具有针对AM靶向治疗方式的临床前评估潜力。
Ameloblastomas are odontogenic tumors that are rare in people but have a relatively high prevalence in dogs. Because canine acanthomatous ameloblastomas (CAA) have clinicopathologic and molecular features in common with human ameloblastomas (AM), spontaneous CAA can serve as a useful translational model of disease. However, the molecular basis of CAA and how it compares to AM are incompletely understood. In this study, we compared the global genomic expression profile of CAA with AM and evaluated its dental origin by using a bulk RNA-seq approach. For these studies, healthy gingiva and canine oral squamous cell carcinoma served as controls. We found that aberrant RAS signaling, and activation of the epithelial-to-mesenchymal transition cellular program are involved in the pathogenesis of CAA, and that CAA is enriched with genes known to be upregulated in AM including those expressed during the early stages of tooth development, suggesting a high level of molecular homology. These results support the model that domestic dogs with spontaneous CAA have potential for pre-clinical assessment of targeted therapeutic modalities against AM.
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DOI：
10.1016/s1079-2104(05)80104-0
发表时间：
1995-05-01
影响因子：
--
作者：
GARDNER, DG
通讯作者：
GARDNER, DG
影响因子：
1
作者：
Goldschmidt, Stephanie L.;Bell, Cindy M.;Soukup, Jason
通讯作者：
Soukup, Jason
DOI：
10.1016/j.neo.2020.10.003
发表时间：
2020-12
期刊：
Neoplasia (New York, N.Y.)
影响因子：
--
作者：
Guscetti F;Nassiri S;Beebe E;Rito Brandao I;Graf R;Markkanen E
通讯作者：
Markkanen E
影响因子：
7.6
作者：
Heikinheimo, K;Jee, KJ;Knuutila, S
通讯作者：
Knuutila, S
DOI：
10.1083/jcb.201512074
发表时间：
2016-09-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ahtiainen L;Uski I;Thesleff I;Mikkola ML
通讯作者：
Mikkola ML