Cell-cycle-specific Cellular Responses to Sonoporation.
Cell-cycle-specific Cellular Responses to Sonoporation.
复制标题
细胞周期特异性细胞对声孔反应的反应
DOI：
10.7150/thno.20820
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发表时间：
2017
期刊：
影响因子：
12.4
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Fan P;Zhang Y;Guo X;Cai C;Wang M;Yang D;Li Y;Tu J;Crum LA;Wu J;Zhang D
微泡介导的声致穿孔在促进基因/药物和其他难以进入细胞的治疗剂的细胞内摄取方面显示出巨大的潜力。然而，微泡-细胞相互作用的生物物理机制仍不清楚。特别是，它仍然是一个重大的挑战，以获得一个全面的了解细胞周期时相的影响，同时发生在细胞膜和细胞骨架微泡声致孔诱导的细胞反应。方法：在这里，进行有效的同步化，以阻止人宫颈上皮癌（HeLa）细胞在各个周期阶段。使用原子力显微镜检查同步化细胞的表面结构和刚度。利用实时荧光成像系统同时分析了声致穿孔引起的细胞膜透性和细胞骨架排列的变化。结果如下：结果表明，G1期细胞的高度和弹性模量最大，而S期细胞通常最平坦和最柔软。因此，S期被认为是瞬时声孔处理的优选周期，这是由于在声孔处理后的早期阶段膜渗透性的最大增强和最快的细胞骨架解体。结论：目前的研究结果可能有利于正在进行的努力，旨在追求合理利用微泡介导的声孔在细胞周期靶向基因/药物递送癌症治疗。
Microbubble-mediated sonoporation has shown its great potential in facilitating intracellular uptake of gene/drugs and other therapeutic agents that are otherwise difficult to enter cells. However, the biophysical mechanisms underlying microbubble-cell interactions remain unclear. Particularly, it is still a major challenge to get a comprehensive understanding of the impact of cell cycle phase on the cellular responses simultaneously occurring in cell membrane and cytoskeleton induced by microbubble sonoporation. Methods: Here, efficient synchronizations were performed to arrest human cervical epithelial carcinoma (HeLa) cells in individual cycle phases. The, topography and stiffness of synchronized cells were examined using atomic force microscopy. The variations in cell membrane permeabilization and cytoskeleton arrangement induced by sonoporation were analyzed simultaneously by a real-time fluorescence imaging system. Results: The results showed that G1-phase cells typically had the largest height and elastic modulus, while S-phase cells were generally the flattest and softest ones. Consequently, the S-Phase was found to be the preferred cycle for instantaneous sonoporation treatment, due to the greatest enhancement of membrane permeability and the fastest cytoskeleton disassembly at the early stage after sonoporation. Conclusion: The current findings may benefit ongoing efforts aiming to pursue rational utilization of microbubble-mediated sonoporation in cell cycle-targeted gene/drug delivery for cancer therapy.
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影响因子：
4.2
作者：
DEJONG, N;CORNET, R;LANCEE, CT
通讯作者：
LANCEE, CT
影响因子：
2.9
作者：
Hu, Yaxin;Wan, Jennifer M. F.;Yu, Alfred C. H.
通讯作者：
Yu, Alfred C. H.
影响因子：
10.8
作者：
Geers, Bart;Dewitte, Heleen;Lentacker, Ine
通讯作者：
Lentacker, Ine
影响因子：
10.8
作者：
De Cock, Ine;Zagato, Elisa;Lentacker, Ine
通讯作者：
Lentacker, Ine
影响因子：
3.5
作者：
Bentes de Paula, Daisy Maria;Valero-Lapchik, Valderez Bastos;Han, Sang Won
通讯作者：
Han, Sang Won