Tim-3 expression in cervical cancer promotes tumor metastasis.
Tim-3 expression in cervical cancer promotes tumor metastasis.
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Tim-3在宫颈癌中的表达促进肿瘤转移
DOI：
10.1371/journal.pone.0053834
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Cao Y;Zhou X;Huang X;Li Q;Gao L;Jiang L;Huang M;Zhou J
研究背景T细胞免疫球蛋白粘蛋白3（Tim-3）是抗肿瘤免疫的负性调节因子。最近的研究强调了Tim-3在人类和动物癌症模型中发生的CD 8 + T细胞耗竭中的重要作用。然而，Tim-3在肿瘤细胞中表达的性质及其抑制抗肿瘤免疫的机制尚不清楚。本研究旨在确定Tim-3在宫颈癌细胞中的表达，并评估Tim-3在宫颈癌进展中的作用。方法收集85例宫颈组织标本，其中宫颈癌43例，宫颈上皮内瘤样病变（CIN）22例，慢性宫颈炎20例。检测肿瘤细胞中Tim-3的表达，发现其与临床病理参数相关。同时采用RT-PCR、Western Blot和共聚焦显微镜检测宫颈癌细胞系HeLa和SiHa中Tim-3的表达。通过ADV反义Tim-3抑制Tim-3表达后，评估Hela细胞的迁移和侵袭能力。结论Tim-3在临床宫颈癌组织中的表达高于CIN和慢性宫颈炎组织。我们通过证实宫颈细胞系中存在Tim-3 mRNA和蛋白质来支持这一发现。肿瘤细胞中Tim-3的表达与临床病理参数相关。Tim-3高表达的患者比低表达的患者具有显著的转移潜能、晚期癌症分级和较短的总生存期。多因素分析显示Tim-3表达是预测宫颈癌预后的独立因素。下调Tim-3蛋白表达可明显抑制Hela细胞的迁移和侵袭。我们的研究表明肿瘤细胞中Tim-3的表达可能是宫颈癌患者的独立预后因素。此外，Tim-3表达可能促进宫颈癌的转移潜力。
Background T cell immunoglobulin mucin-3 (Tim-3) has been identified as a negative regulator of anti-tumor immunity. Recent studies highlight the important role of Tim-3 in the CD8+ T cell exhaustion that takes place in both human and animal cancer models. However, the nature of Tim-3 expression in the tumor cell and the mechanism by which it inhibits anti-tumor immunity are unclear. This present study aims to determine Tim-3 is expressed in cervical cancer cells and to evaluate the role of Tim-3 in cervical cancer progression. Methodology A total of 85 cervical tissue specimens including 43 human cervical cancer, 22 cervical intraepithelial neoplasia (CIN) and 20 chronic cervicitis were involved. Tim-3 expression in tumor cells was detected and was found to correlate with clinicopathological parameters. Meanwhile, expression of Tim-3 was assessed by RT-PCR, Western Blot and confocal microscopy in cervical cancer cell lines, HeLa and SiHa. The migration and invasion potential of Hela cells was evaluated after inhibiting Tim-3 expression by ADV-antisense Tim-3. Conclusions We found that Tim-3 was expressed at a higher level in the clinical cervical cancer cells compared to the CIN and chronic cervicitis controls. We supported this finding by confirming the presence of Tim-3 mRNA and protein in the cervical cell lines. Tim-3 expression in tumor cells correlated with clinicopathological parameters. Patients with high expression of Tim-3 had a significant metastatic potential, advanced cancer grades and shorter overall survival than those with lower expression. Multivariate analysis showed that Tim-3 expression was an independent factor for predicting the prognosis of cervical cancer. Significantly, down-regulating the expression of Tim-3 protein inhibited migration and invasion of Hela cells. Our study suggests that the expression of Tim-3 in tumor cells may be an independent prognostic factor for patients with cervical cancer. Moreover, Tim-3 expression may promote metastatic potential in cervical cancers.
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影响因子：
16.8
作者：
Sakuishi K;Jayaraman P;Behar SM;Anderson AC;Kuchroo VK
通讯作者：
Kuchroo VK
影响因子：
6.2
作者：
Ibrahim, R;Frederickson, H;Khleif, SN
通讯作者：
Khleif, SN
影响因子：
9.7
作者：
Tu, Bing;Du, Lin;Tang, Ting-Ting
通讯作者：
Tang, Ting-Ting
影响因子：
11.5
作者：
Zhou, JF;Gao, QL;Ma, D
通讯作者：
Ma, D
淋巴瘤内皮优先表达 Tim-3 并通过介导免疫逃避促进淋巴瘤的进展
DOI：
10.1084/jem.20090397
发表时间：
2010-03-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Huang X;Bai X;Cao Y;Wu J;Huang M;Tang D;Tao S;Zhu T;Liu Y;Yang Y;Zhou X;Zhao Y;Wu M;Wei J;Wang D;Xu G;Wang S;Ma D;Zhou J
通讯作者：
Zhou J