Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans.
Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans.
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DOI：
10.3390/ijms23137202
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发表时间：
2022-06-29
影响因子：
5.6
通讯作者：
Wang, Bin
中科院分区：
文献类型：
--
作者：
Odiba, Arome Solomon;Ezechukwu, Chiemekam Samuel;Liao, Guiyan;Li, Siqiao;Chen, Zhongliang;Liu, Xihui;Fang, Wenxia;Jin, Cheng;Wang, Bin
染色体结构维持复合体（Structural Maintenance of Chromosomes，SMC）在维持染色体完整性方面发挥着重要作用，其中SMC 5/6复合体在有丝分裂和减数分裂过程以及DNA修复中占据中心地位。NSE-4 Kleisin对于SMC 5/6复合物的组织和功能都是至关重要的，将NSE 1和NSE 3（法师相关）与SMC 5和SMC 6蛋白的头部结构域桥接。尽管保守的蛋白质序列，没有功能相关的NSE-4同源蛋白（NSE-4）在秀丽隐杆线虫的报道。在这里，我们展示了C的重要作用。NSE-4在基因组维持和DNA修复中的作用我们的研究结果表明，NSE-4是维持染色体结构和修复一系列化学诱导的DNA损伤所必需的。此外，NSE-4还参与减数分裂期间的姐妹间修复。NSE-4定位于染色体上，并且对于NSE-1的定位是不可或缺的。总的来说，我们从这项研究的数据提供了进一步的洞察进化的保守性和多样化的NSE-4功能的SMC-5/6复合物。
The Structural Maintenance of Chromosomes (SMC) complex plays an important role in maintaining chromosome integrity, in which the SMC5/6 complex occupies a central position by facilitating mitotic and meiotic processes as well as DNA repair. NSE-4 Kleisin is critical for both the organization and function of the SMC5/6 complex, bridging NSE1 and NSE3 (MAGE related) with the head domains of the SMC5 and SMC6 proteins. Despite the conservation in protein sequence, no functional relevance of the NSE-4 homologous protein (NSE-4) in Caenorhabditis elegans has been reported. Here, we demonstrated the essential role of C. elegans NSE-4 in genome maintenance and DNA repair. Our results showed that NSE-4 is essential for the maintenance of chromosomal structure and repair of a range of chemically induced DNA damage. Furthermore, NSE-4 is involved in inter-sister repair during meiosis. NSE-4 localizes on the chromosome and is indispensable for the localization of NSE-1. Collectively, our data from this study provide further insight into the evolutionary conservation and diversification of NSE-4 function in the SMC-5/6 complex.
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影响因子：
4.5
作者：
Hong Y;Sonneville R;Agostinho A;Meier B;Wang B;Blow JJ;Gartner A
通讯作者：
Gartner A
影响因子：
4.5
作者：
Bailly AP;Freeman A;Hall J;Déclais AC;Alpi A;Lilley DM;Ahmed S;Gartner A
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Gartner A
影响因子：
19
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通讯作者：
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影响因子：
3.7
作者：
Guerineau M;Kriz Z;Kozakova L;Bednarova K;Janos P;Palecek J
通讯作者：
Palecek J
影响因子：
11.8
作者：
Colaiácovo, MP;MacQueen, AJ;Villeneuve, AM
通讯作者：
Villeneuve, AM