Enhancement of the incorporation of 5-fluorodeoxyuridylate into DNA of HL-60 cells by metabolic modulations.
Enhancement of the incorporation of 5-fluorodeoxyuridylate into DNA of HL-60 cells by metabolic modulations.
复制标题
通过代谢调节增强 5-氟脱氧尿苷酸掺入 HL-60 细胞的 DNA。
作者：
M. Tanaka;K. Kimura;S. Yoshida
将 HL-60 人早幼粒细胞白血病细胞暴露于 0.5 microM 5-氟-2'-[3H]脱氧尿苷 (FdUrd) 16 小时，导致每摩尔 DNA 核苷酸将 5.14 +/- 0.31 (S.D.) X 10(-7) mol FdUrd 掺入 DNA，相当于 0.146 +/-每 10(7) 个细胞 0.082 pmol FdUrd。用 50 µM 脱氧胸苷预处理 24 小时，在随后暴露于 0.5 µM [3H]FdUrd 的 16 小时期间，该类似物与新合成 DNA 的掺入量增加了 2.7 倍。用 0.5 microM 甲氨蝶呤预处理 3 小时也使新合成 DNA 中的 [3H]FdUrd 掺入量增加了约 5 倍。然而，脱氧胸苷或甲氨蝶呤与[3H]FdUrd的共存导致FdUrd掺入DNA的量减少。通过 Cs2SO4 平衡密度梯度离心分离的 DNA 中超过 50% 的放射性通过其与干酪乳杆菌脱氧胸苷单磷酸合成酶的结合被证明是氟脱氧尿苷酸。
The exposure of HL-60 human promyelocytic leukemia cells to 0.5 microM 5-fluoro-2'-[3H]deoxyuridine (FdUrd) for 16 hr resulted in the incorporation of 5.14 +/- 0.31 (S.D.) X 10(-7) mol FdUrd into DNA per mol of DNA nucleotide, which corresponds to 0.146 +/- 0.082 pmol FdUrd per 10(7) cells. Pretreatment with 50 microM deoxythymidine for 24 hr led to a 2.7-fold increase in the incorporation of this analogue into newly synthesized DNA during the ensuing 16-hr exposure to 0.5 microM [3H]FdUrd. Pretreatment with 0.5 microM methotrexate for 3 hr also increased the [3H]FdUrd incorporation into newly synthesized DNA approximately 5-fold. The coexistence of deoxythymidine or methotrexate with [3H]FdUrd, however, led to decreased incorporation of FdUrd into DNA. More than 50% of the radioactivity in DNA separated by Cs2SO4 equilibrium density gradient centrifugation was proven to be fluorodeoxyuridylate by means of its binding to Lactobacillus casei deoxythymidine monophosphate synthetase.
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影响因子：
11.2
作者：
P. Major;E. Egan;D. Herrick;D. Kufe
通讯作者：
P. Major;E. Egan;D. Herrick;D. Kufe
甲氨蝶呤对人乳腺癌 DNA 中 5-氟尿嘧啶残基掺入和切除的影响。
影响因子：
11.2
作者：
Herrick,DJ;Major,PP;Kufe,DW
通讯作者：
Kufe,DW
影响因子：
14.9
作者：
Pettersen HS;Visnes T;Vågbø CB;Svaasand EK;Doseth B;Slupphaug G;Kavli B;Krokan HE
通讯作者：
Krokan HE
5-氟-2-脱氧尿苷掺入 L1210 DNA 中。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kufe,DW;Major,PP;Egan,EM;Loh,E
通讯作者：
Loh,E