Woronin body hitchhiking on early endosomes is dispensable for septal localization in Aspergillus nidulans.
Woronin body hitchhiking on early endosomes is dispensable for septal localization in Aspergillus nidulans.
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DOI：
10.1091/mbc.e23-01-0025
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发表时间：
2023-06-01
影响因子：
3.3
通讯作者：
Reck-Peterson, Samara L.
中科院分区：
文献类型：
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作者：
Songster, Livia D.;Bhuyan, Devahuti;Christensen, Jenna R.;Reck-Peterson, Samara L.
细胞器的正常功能取决于它们的细胞内定位，由细胞骨架轨道上的运动蛋白依赖性运输介导。在丝状真菌构巢曲霉中，过氧化物酶体不是直接与马达蛋白结合，而是通过搭便车在运动的早期内体上移动。然而，过氧化物酶体搭便车的生理作用尚不清楚。过氧化物酶体搭便车需要蛋白质PxdA，这是保守的真菌亚门盘菌，但缺乏其他真菌分支。Woronin体是盘菌亚门特有的过氧化物酶体。在这些真菌中，多核菌丝节被称为隔膜的不完整细胞壁分隔，隔膜具有能够进行细胞质交换的中心孔。当菌丝节受损时，沃罗宁体堵塞隔膜孔以防止广泛渗漏。在这里，我们测试了过氧化物酶体搭便车是否对A. nidulans。我们发现，Woronin体蛋白存在于所有能动的过氧化物酶体和搭便车的PxdA标记的早期内涵体在双向，长距离的运动。过氧化物酶体搭便车的损失显着影响Woronin体在细胞质中的分布和运动性，但Woronin体搭便车最终是由于隔定位和堵塞。
The proper functioning of organelles depends on their intracellular localization, mediated by motor protein–dependent transport on cytoskeletal tracks. Rather than directly associating with a motor protein, peroxisomes move by hitchhiking on motile early endosomes in the filamentous fungus Aspergillus nidulans. However, the physiological role of peroxisome hitchhiking is unclear. Peroxisome hitchhiking requires the protein PxdA, which is conserved within the fungal subphylum Pezizomycotina but absent from other fungal clades. Woronin bodies are specialized peroxisomes that are also unique to the Pezizomycotina. In these fungi, multinucleate hyphal segments are separated by incomplete cell walls called septa that possess a central pore enabling cytoplasmic exchange. Upon damage to a hyphal segment, Woronin bodies plug septal pores to prevent widespread leakage. Here, we tested whether peroxisome hitchhiking is important for Woronin body motility, distribution, and function in A. nidulans. We show that Woronin body proteins are present within all motile peroxisomes and hitchhike on PxdA-labeled early endosomes during bidirectional, long-distance movements. Loss of peroxisome hitchhiking significantly affected Woronin body distribution and motility in the cytoplasm, but Woronin body hitchhiking is ultimately dispensable for septal localization and plugging.
通过过氧化物酶体蛋白排序从一个中制成两个细胞器：Woronin身体生物发生。
影响因子：
7.8
作者：
Liu, Fangfang;Ng, Seng Kah;Lu, Yanfen;Low, Wilson;Lai, Julian;Jedd, Gregory
通讯作者：
Jedd, Gregory
影响因子：
4.5
作者：
Ng SK;Liu F;Lai J;Low W;Jedd G
通讯作者：
Jedd G
MRHEX1是Robertsii Metarhizium Robertsii中Woronin身体形成，真菌发育和毒力所必需的。
DOI：
10.3390/jof6030172
发表时间：
2020-09-14
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
Tang G;Shang Y;Li S;Wang C
通讯作者：
Wang C