MrHex1 is Required for Woronin Body Formation, Fungal Development and Virulence in Metarhizium robertsii.
MrHex1 is Required for Woronin Body Formation, Fungal Development and Virulence in Metarhizium robertsii.
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MRHEX1是Robertsii Metarhizium Robertsii中Woronin身体形成，真菌发育和毒力所必需的。
DOI：
10.3390/jof6030172
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发表时间：
2020-09-14
期刊：
影响因子：
--
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Tang G;Shang Y;Li S;Wang C
Woronin体（WB）是一种过氧化物酶体衍生的致密核心囊泡，是一种自组装的单一蛋白质Hex 1的六边形晶体。这种细胞器是属于盘菌亚门的子囊真菌特有的，其功能是响应于菌丝体损伤和控制细胞异质性而密封隔膜孔。我们从GenBank数据库中检索了所有可用的含有不同真菌的Hex 1结构域的蛋白质，并在460个获得的Hex 1蛋白质中发现了相当大的长度变化。然而，一个高度保守的Hex 1结构域含有75个氨基酸残基，具有特异性的S/A-R/S-L共有基序，用于靶向过氧化物酶体存在于每个蛋白质的羧基末端。昆虫病原真菌罗伯茨绿僵菌（Metarhizium robertsii）的同源Hex 1基因被删除，命名为MrHex 1。MrHex 1基因在M. robertsii，并参与密封隔膜孔以维持细胞完整性和异质性。不同的试验表明，相对于野生型（WT）菌株，Mrhex 1表现出在固体培养基上的生长缺陷和对昆虫宿主的分生孢子，附着胞形成和局部感染性的大幅减少。但在昆虫注射过程中，野生型与突变型的毒力差异不明显。我们还发现MrHex 1可以耐受不同的胁迫条件，如WT和M的基因拯救突变体。robertsii，这与其他真菌物种的Hex 1无效突变体的应激反应缺陷的报道相反。除了揭示不同致病型真菌之间Hex 1缺失突变体的表型/功能改变外，本研究的结果可能有助于理解真菌昆虫致病性的进化和WB控制。
The Woronin body (WB) is a peroxisome-derived dense-core vesicle, a self-assembling hexagonal crystal of a single protein Hex1. This organelle is specific to the ascomycete fungi belonging to the Pezizomycotina subphylum by functioning in sealing septal pores in response to mycelium damage and the control of cell heterogeneity. We retrieved all available Hex1-domain containing proteins of different fungi from the GenBank database and found considerable length variations among 460 obtained Hex1 proteins. However, a highly conserved Hex1 domain containing 75 amino acid residues with a specific S/A-R/S-L consensus motif for targeting peroxisome is present at the carboxy-terminus of each protein. A homologous Hex1 gene, named MrHex1, was deleted in the entomopathogenic fungus Metarhizium robertsii. It was found that MrHex1 was responsible for WB formation in M. robertsii and involved in sealing septal pores to maintain cell integrity and heterogeneity. Different assays indicated that, relative to the wild-type (WT) strain, ∆Mrhex1 demonstrated a growth defect on a solid medium and substantial reductions of conidiation, appressorium formation and topical infectivity against insect hosts. However, there was no obvious virulence difference between WT and mutants during injection of insects. We also found that ∆MrHex1 could tolerate different stress conditions like the WT and the gene-rescued mutant of M. robertsii, which is in contrast to the reports of the stress-response defects of the Hex1 null mutants of other fungal species. In addition to revealing the phenotypic/functional alterations of the Hex1 deletion mutants between different pathotype fungi, the results of this study may benefit the understanding of the evolution and WB-control of fungal entomopathogenicity.
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影响因子：
4.8
作者：
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