Differential expression of mature microRNAs involved in muscle maintenance of hibernating little brown bats, Myotis lucifugus: a model of muscle atrophy resistance.
Differential expression of mature microRNAs involved in muscle maintenance of hibernating little brown bats, Myotis lucifugus: a model of muscle atrophy resistance.
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DOI：
10.1016/j.gpb.2012.09.001
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发表时间：
2012-10
影响因子：
9.5
通讯作者：
Storey, Kenneth B.
中科院分区：
文献类型：
--
作者：
Kornfeld, Samantha F.;Biggar, Kyle K.;Storey, Kenneth B.
在哺乳动物中，肌肉萎缩在长时间的不动中是常见的。然而，许多小型冬眠哺乳动物设法避免肌肉萎缩，尽管在冬眠期间长时间保持静止。最近的研究强调了短的非编码microRNA（miRNAs）在调节胁迫耐受性中的作用。我们提出，它们也可以在冬眠期间的肌肉维护中发挥重要作用。为了探索这种可能性，一组10个已知在非冬眠哺乳动物的骨骼肌中正常表达的miRNA通过RT-PCR在冬眠的小棕蝠，鼠耳蝠lucifugus中进行了分析。然后，我们比较了这些miRNAs在正常体温控制蝙蝠和冬眠蝙蝠中的表达。我们的研究结果表明，与正常体温对照相比，在麻痹蝙蝠胸肌中观察到8种成熟miRNA的转录表达显著增加，尽管幅度不大（1.2-1.6倍），包括miR-1a-1，miR-29 b，miR-181 b，miR-15 a，miR-20 a，miR-206和miR-128-1。相反，在休眠期间，miR-21的表达下降了80%，而miR-107的表达保持不受影响。有趣的是，这些miRNAs已经被验证或预测会影响多种肌肉特异性因子，包括肌肉生长抑制素，FoxO 3a，HDAC 4和SMAD 7，并且可能参与蝙蝠冬眠期间胸肌质量和功能的保存。
Muscle wasting is common in mammals during extended periods of immobility. However, many small hibernating mammals manage to avoid muscle atrophy despite remaining stationary for long periods during hibernation. Recent research has highlighted roles for short non-coding microRNAs (miRNAs) in the regulation of stress tolerance. We proposed that they could also play an important role in muscle maintenance during hibernation. To explore this possibility, a group of 10 miRNAs known to be normally expressed in skeletal muscle of non-hibernating mammals were analyzed by RT-PCR in hibernating little brown bats, Myotis lucifugus. We then compared the expression of these miRNAs in euthermic control bats and bats in torpor. Our results showed that compared to euthermic controls, significant, albeit modest (1.2–1.6 fold), increases in transcript expression were observed for eight mature miRNAs, including miR-1a-1, miR-29b, miR-181b, miR-15a, miR-20a, miR-206 and miR-128-1, in the pectoral muscle of torpid bats. Conversely, expression of miR-21 decreased by 80% during torpor, while expression of miR-107 remained unaffected. Interestingly, these miRNAs have been either validated or predicted to affect multiple muscle-specific factors, including myostatin, FoxO3a, HDAC4 and SMAD7, and are likely involved in the preservation of pectoral muscle mass and functionality during bat hibernation.
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影响因子：
16
作者：
Leung AK;Sharp PA
通讯作者：
Sharp PA
DOI：
10.1073/pnas.1014523107
发表时间：
2010-11-02
影响因子：
11.1
作者：
Andrews, Jessica L.;Zhang, Xiping;Esser, Karyn A.
通讯作者：
Esser, Karyn A.
影响因子：
5.6
作者：
Lee, Kisoo;So, Hyekyoung;Choi, Inho
通讯作者：
Choi, Inho
对骨骼肌生长不同的鸡肉品种的骨骼肌 miRNA 转录组进行系统分析，鉴定出新的 miRNA 和差异表达的 miRNA
影响因子：
4.4
作者：
Li T;Wu R;Zhang Y;Zhu D
通讯作者：
Zhu D
影响因子：
3.6
作者：
Eddy, Sean F.;Storey, Kenneth B.
通讯作者：
Storey, Kenneth B.