Menisci protect chondrocytes from load-induced injury.
Menisci protect chondrocytes from load-induced injury.
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DOI：
10.1038/s41598-018-32503-1
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发表时间：
2018-09-20
影响因子：
4.6
通讯作者：
Herzog W
中科院分区：
文献类型：
--
作者：
Abusara Z;Andrews SHJ;Von Kossel M;Herzog W
膝关节中的半月板被认为提供了稳定性，增加了接触面积，降低了接触压力，并在关节负荷过程中保护了底层的关节软骨和骨骼。半月板的丢失或损伤通常伴随着膝关节的退行性变化，导致包括人类在内的动物患骨性关节炎的风险增加。然而，关节退变和骨关节炎发生的详细机制仍然很大程度上是未知的，半月板丢失的急性影响也没有得到系统的研究。我们开发了一个基于显微镜的系统来研究受控肌肉收缩负荷下的活小鼠的微观关节力学。在这里，我们展示了半月板丢失如何与关节软骨损伤后数小时内软骨细胞的迅速死亡(坏死)有关，以及完整的半月板如何在存在强烈的肌肉关节负荷和/或关节软骨损伤的情况下保护体内的软骨细胞。我们的研究结果表明，半月板丢失后的膝关节负荷与完整和受伤的膝关节中广泛的细胞死亡有关，早期治疗干预的目的应该是防止软骨细胞死亡。
Menisci in the knee joint are thought to provide stability, increased contact area, decreased contact pressures, and offer protection to the underlying articular cartilage and bone during joint loading. Meniscal loss or injury is typically accompanied by degenerative changes in the knee, leading to an increased risk for osteoarthritis in animals including humans. However, the detailed mechanisms underlying joint degeneration and the development of osteoarthritis remain largely unknown, and the acute effects of meniscal loss have not been studied systematically. We developed a microscopy-based system to study microscale joint mechanics in living mice loaded by controlled muscular contractions. Here, we show how meniscal loss is associated with rapid chondrocyte death (necrosis) in articular cartilage within hours of injury, and how intact menisci protect chondrocytes in vivo in the presence of intense muscle-based joint loading and/or injury to the articular cartilage. Our findings suggest that loading the knee after meniscal loss is associated with extensive cell death in intact and injured knees, and that early treatment interventions should be aimed at preventing chondrocyte death.
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影响因子：
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DOI：
10.1302/0301-620x.30b4.664
发表时间：
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