HtrA‐dependent adherence and invasion of Campylobacter jejuni in human vs avian cells
HtrA‐dependent adherence and invasion of Campylobacter jejuni in human vs avian cells
复制标题
空肠弯曲杆菌在人类和禽类细胞中依赖于 HtrA 的粘附和侵袭
DOI：
10.1111/lam.13277
复制
发表时间：
2019
影响因子：
2.4
通讯作者：
Backert S
中科院分区：
文献类型：
--
作者：
Simson D;Boehm M;Backert S
本研究的目的是研究HtrA是否是空肠弯曲菌对人和鸡细胞系粘附和侵袭差异的原因。使用C对人Caco-2或鸡2G 4细胞进行庆大霉素保护试验。jejunistrain NCTC 11168来比较对这两种细胞类型的粘附和侵袭率。结果显示，人和禽类细胞之间的粘附和侵袭率存在显着差异。缺失弯曲杆菌htrA基因，编码丝氨酸蛋白酶和伴侣蛋白的双重功能，在发病机制中发挥作用，导致两种细胞系中的比率降低。使用缺乏蛋白酶活性但保留伴侣蛋白活性的单氨基酸取代突变体（ΔhtrA/htrAS 197 A），我们表明第一个参与了人Caco-2和鸡2G 4细胞的侵袭，而后者突变体的侵袭水平较低。对鸡细胞的粘附性高于对Caco-2细胞的粘附性，这也依赖于HtrA。总之，这些数据表明HtrA的蛋白水解活性参与了宿主对C.空肠弯曲菌是全球细菌性食源性小肠结肠炎的主要原因。虽然人类肠道的定殖可能导致严重的问题，但作为主要感染源的禽类宿主仍然不受细菌的影响。我们发现，细菌丝氨酸蛋白酶和伴侣蛋白HtrA参与的粘附和入侵在这两个物种之间的不同宿主的毒力的差异不负责。将来，HtrA可能作为抑制剂的靶标，以避免或消除鸡中的定植，作为商业畜牧业中抗生素的一种问题较少的替代品。
The aim of this study was to investigate whether HtrA is responsible for differences in adherence and invasion ofCampylobacter jejunitowards human and chicken cell lines. Gentamicin protection assays were performed with either human Caco‐2 or chicken 2G4 cells usingC. jejunistrain NCTC11168 to compare the adhesion and invasion rates towards these two cell types. The results revealed significant differences in the adhesion and invasion rates between the human and avian cells. Deletion of theCampylobacter htrAgene, coding for the dual function of serine protease and chaperonin with a role in pathogenesis, led to a reduction of the rates in both cell lines. Using a single‐amino acid substitution mutant (ΔhtrA/htrAS197A) that lacked protease activity, but retained chaperonin activity, we show that the first is involved in the invasion of human Caco‐2 and chicken 2G4 cells, whereas the latter mutant invaded at lower levels. Adherence towards the chicken cells is higher than towards Caco‐2 cells and this is also dependent on HtrA. Together, these data suggest that the proteolytic activity of HtrA is involved in the difference in host response ofC. jejunitowards human and chicken‐derived cells.Significance and Impact of the StudyCampylobacter jejuniis the main cause for bacterial foodborne enterocolitis worldwide. While colonization of the human intestine can lead to severe problems, avian hosts – as the major source of infection – remain unaffected by the bacteria. We showed that the bacterial serine protease and chaperonin HtrA are involved in adhesion and invasion in both species and not responsible for the discrepancy of virulence between the different hosts. In future, HtrA might act as a target for inhibitors to avoid or eradicate colonization in chickens as a less problematic alternative to antibiotics in commercial livestock breeding.
DOI：
10.1186/1478-811x-11-72
发表时间：
2013-09-30
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
Backert S;Boehm M;Wessler S;Tegtmeyer N
通讯作者：
Tegtmeyer N