MAPK docking motif in the Dictyostelium Gα2 subunit is required for aggregation and transcription factor translocation.
MAPK docking motif in the Dictyostelium Gα2 subunit is required for aggregation and transcription factor translocation.
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DOI：
10.1016/j.cellsig.2021.110117
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发表时间：
2021-11
影响因子：
4.8
通讯作者：
Hadwiger JA
中科院分区：
文献类型：
--
作者：
Adhikari N;McGill IN;Hadwiger JA
一些G蛋白α亚基在氨基末端附近含有丝裂原活化蛋白激酶（MAPK）对接基序（D-motif），其可以影响细胞对外部信号的反应。Dictyosteoprotein Gα2 G蛋白亚基在多细胞发育开始时对cAMP的趋化性是必需的，该亚基在氨基末端附近含有一个推定的D基序。改变Gα2亚基D-基序以检查其在趋化性和多细胞发育中的潜在作用。在gα2−细胞中，来自高拷贝数载体的D基序突变体（Gα2D−）或野生型亚基的表达拯救了细胞聚集，但阻止了土丘向蛞蝓的转变。在具有野生型gα2基因座的亲本菌株中也观察到这种表型，表明异源Gα2亚基表达干扰多细胞形态发生。在gα2−细胞中，来自低拷贝数载体的Gα2D−亚基的表达不能拯救聚集，而野生型Gα2亚基有效地拯救了聚集，并允许野生型形态发育。Gα2D−和Gα2亚基都能够恢复类似水平的cAMP刺激的运动性和与野生型细胞共聚集的能力，这意味着Gα2D−表达细胞的聚集缺陷是由于细胞间信号传导不足。Gα2亚基而非Gα2D−亚基的表达完全恢复了cAMP刺激GtaC转录因子易位的能力，表明D基序对转录因子调控很重要。这些结果表明Gα2的D基序在聚集和其他涉及cAMP信号传导的发育反应中起作用。
Some G protein alpha subunits contain a mitogen-activated protein kinase (MAPK) docking motif (D-motif) near the amino terminus that can impact cellular responses to external signals. The Dictyostelium Gα2 G protein subunit is required for chemotaxis to cAMP during the onset of multicellular development and this subunit contains a putative D-motif near the amino terminus. The Gα2 subunit D-motif was altered to examine its potential role in chemotaxis and multicellular development. In gα2− cells the expression of the D-motif mutant (Gα2D−) or wild-type subunit from high copy number vectors rescued cell aggregation but blocked the transition of mounds into slugs. This phenotype was also observed in parental strains with a wild-type gα2 locus indicating that the heterologous Gα2 subunit expression interferes with multicellular morphogenesis. Expression of the Gα2D− subunit from a low copy number vectors in gα2− cells did not rescue aggregation whereas the wild-type Gα2 subunit rescued aggregation efficiently and allowed wild-type morphological development. The Gα2D− and Gα2 subunit were both capable of restoring comparable levels of cAMP stimulated motility and the ability to co-aggregate with wild-type cells implying that the aggregation defect of Gα2D− expressing cells is due to insufficient intercellular signaling. Expression of the Gα2 subunit but not the Gα2D− subunit fully restored the ability of cAMP to stimulate the translocation of the GtaC transcription factor suggesting the D-motif is important for transcription factor regulation. These results suggest that the D-motif of Gα2 plays a role in aggregation and other developmental responses involved with cAMP signaling.
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影响因子：
8
作者：
Artemenko, Yulia;Lampert, Thomas J.;Devreotes, Peter N.
通讯作者：
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影响因子：
5.3
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影响因子：
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影响因子：
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影响因子：
3.7
作者：
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通讯作者：
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