Role of UV-damaged DNA-binding protein-1 (DDB1) in early response to Pseudomonas syringae pv. tomato DC3000 infection in tomato
Role of UV-damaged DNA-binding protein-1 (DDB1) in early response to Pseudomonas syringae pv. tomato DC3000 infection in tomato
复制标题
紫外线损伤的 DNA 结合蛋白 1 (DDB1) 在对丁香假单胞菌 pv. 的早期反应中的作用。
DOI：
10.1080/07060661.2018.1518930
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发表时间：
2018-09
影响因子：
2
通讯作者：
Liu Yongsheng
中科院分区：
文献类型：
--
作者：
Zhang Danfeng;Yue Junyang;Hua Chenyan;Chen Danyang;Han Yi;Liu Yongsheng
摘要病原菌引起的病害是影响作物产量和品质的最重要的胁迫因子之一，植物对病原菌的侵染已经形成了一个复杂的反应网络。番茄紫外线损伤DNA结合蛋白1（DDB 1）在番茄抗农杆菌感染中起重要作用。然而，DDB 1蛋白对植物抗病性的影响在很大程度上仍然未知。丁香假单胞菌番茄DC 3000（Pst DC 3000）是番茄的重要病原菌，已被用作感病性测定的模型。在这项工作中，我们比较了野生型幼苗AC+，DDB 1缺陷突变体（hp 1）和DDB 1过表达（DDB 1-OE）幼苗之间的敏感性。结果表明，hp 1突变体对Pst DC 3000更敏感，而DDB 1-OE幼苗的抗性增强。过氧化氢，植物防御反应和相应的细胞死亡表型的关键组成部分受到损害的hp 1突变体和DDB 1-OE植物增强。与此相一致，水杨酸生产和PR 1a 1基因表达的DDB 1基因后，感染Pst DC 3000在接种后24小时的正调控。其他病程相关基因（Gras 2，Lrr 22，Pti 5，pky 28和丝裂原活化蛋白激酶3）也诱导DDB 1-OE植物，但损害不同程度的hp 1突变体。总的来说，这项研究表明，DDB 1可能通过调节SA相关的信号通路在番茄防御反应中发挥调节作用。
Abstract Diseases caused by pathogens are one of the most important stress factors that affect crop yield and quality and plants have evolved a complex network of responses to infection. Tomato UV-damaged DNA-binding protein-1 (DDB1) has been implicated in playing a role in resistance against Agrobacterium infection. However, the effect of DDB1 protein on plant disease resistance is still largely unknown. Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) is an important pathogen of tomato and has been used as a model for testing disease susceptibility. In this work, we compared the sensitivity to Pst DC3000 among wild-type seedlings AC+, DDB1-deficient mutants (hp1) and DDB1 over-expression (DDB1-OE) seedlings. Results showed that the hp1 mutant is more susceptible to Pst DC3000, whereas the resistance was increased in the DDB1-OE seedlings. Hydrogen peroxide, the key component of the plant defence response and the corresponding cell death phenotype were compromised in the hp1 mutant and enhanced in the DDB1-OE plants. Consistent with that, salicylic acid production and PR1a1 gene expression were positively regulated by the DDB1 gene upon infection with Pst DC3000 at 24 h after inoculation. Other pathogenesis-related genes (Gras2, Lrr22, Pti5, Wrky28 and mitogen-activated protein kinase 3) were also induced in the DDB1-OE plants but compromised to different degrees in the hp1 mutant. Overall, this study suggests that DDB1 plays a regulatory role in tomato defence responses likely through modulating SA-associated signalling pathways.
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影响因子：
2.8
作者：
Youxin Yang;G. Ahammed;Cai-jun Wu;Shuying Fan;Yan-hong Zhou
通讯作者：
Youxin Yang;G. Ahammed;Cai-jun Wu;Shuying Fan;Yan-hong Zhou
ROS介导的MAPK信号传导在非生物和生物应力引起的相似性和差异中。
影响因子：
5.6
作者：
Jalmi SK;Sinha AK
通讯作者：
Sinha AK
番茄紫外线损伤 DNA 结合蛋白 1 (DDB1) 通过表观遗传方式在器官大小控制中的作用
影响因子：
3.7
作者：
Liu J;Tang X;Gao L;Gao Y;Li Y;Huang S;Sun X;Miao M;Zeng H;Tian X;Niu X;Zheng L;Giovannoni J;Xiao F;Liu Y
通讯作者：
Liu Y
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
4.3
作者：
A. Enyedi
通讯作者：
A. Enyedi