A role of tomato UV-damaged DNA binding protein 1 (DDB1) in organ size control via an epigenetic manner.
A role of tomato UV-damaged DNA binding protein 1 (DDB1) in organ size control via an epigenetic manner.
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番茄紫外线损伤 DNA 结合蛋白 1 (DDB1) 通过表观遗传方式在器官大小控制中的作用
DOI：
10.1371/journal.pone.0042621
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Liu J;Tang X;Gao L;Gao Y;Li Y;Huang S;Sun X;Miao M;Zeng H;Tian X;Niu X;Zheng L;Giovannoni J;Xiao F;Liu Y
表观遗传修饰通常是指通过DNA序列变化以外的机制引起的表型变化，在发育过程中起着重要作用。在这项研究中，我们发现一种选择性剪接的番茄DDB1转录物DDB1F的过表达导致器官大小的减小，DDB1F通常存在于所有测试组织中。从一个强大的花椰菜花叶病毒（CaMV）35S启动子组成型表达DDB1F的转基因植物显示适度减少的大小在营养器官（叶和茎）和生殖器官（花，种子和果实）的大小从根本上减少，其中几个基因编码负调节细胞分裂上调。值得注意的是，由DDB1F转基因过表达所赋予的器官大小的减小似乎在随后的世代中不分离，这表明表型改变以表观遗传方式被操纵并且可以在世代中传递。通过分析SlWEE 1基因（编码细胞分裂的负调节因子）的DNA甲基化水平进一步证实了这一观点，揭示了启动子区域的甲基化减少与该基因表达水平升高之间的相关性。因此，我们的研究结果表明，DDB1在调控参与器官发生的基因的表观遗传状态中起着重要作用，尽管其潜在的机制仍有待阐明。
Epigenetic modification generally refers to phenotypic changes by a mechanism other than changes in DNA sequence and plays a significant role in developmental processes. In this study, we found that overexpression of one alternatively spliced tomato DDB1 transcript, DDB1F that is prevalently present in all tested tissues, resulted in reduction of organ size. Transgenic plants constitutively expressing the DDB1F from a strong cauliflower mosaic virus (CaMV) 35S promoter displayed moderately reduced size in vegetative organs (leaves and stems) and radically decreased size in reproductive organs (flowers, seeds and fruits), in which several genes encoding negative regulators for cell division were upregulated. Significantly, reduction of organ size conferred by overexpression of DDB1F transgene appears not to segregate in the subsequent generations, suggesting the phenotypic alternations are manipulated in an epigenetic manner and can be transmitted over generations. This notion was further substantiated by analysis of DNA methylation level at the SlWEE1 gene (encoding a negative regulator of cell division), revealing a correlation between less methylation in the promoter region and elevated expression level of this gene. Thus, our results suggest DDB1 plays an important role in regulation of the epigenetic state of genes involved in organogenesis, despite the underlying mechanism remains to be elucidated.
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影响因子：
11.2
作者：
Kotake Y;Zeng Y;Xiong Y
通讯作者：
Xiong Y
DOI：
10.1016/j.bbagrm.2011.03.007
发表时间：
2011-08
影响因子：
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作者：
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通讯作者：
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作者：
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通讯作者：
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