Salvage of 5'-deoxy-5'-methylthioadenosine and L-homocysteine into methionine in cells cultured in a methionine-free medium: a study of "methionine-dependence".
Salvage of 5'-deoxy-5'-methylthioadenosine and L-homocysteine into methionine in cells cultured in a methionine-free medium: a study of "methionine-dependence".
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在无蛋氨酸培养基中培养的细胞中将 5-脱氧-5-甲硫腺苷和 L-高半胱氨酸转化为蛋氨酸：“蛋氨酸依赖性”的研究。
DOI：
10.1016/0006-291x(86)90952-6
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发表时间：
1986
影响因子：
3.1
通讯作者：
J. Pérignon
中科院分区：
文献类型：
--
作者：
L. Christa;J. Kersual;J. Augé;J. Pérignon
在以下哺乳动物细胞系Raji、CCL 39和BHK细胞中，比较了两种蛋氨酸前体l -同型半胱氨酸和5′-脱氧-5′-甲基硫腺苷在无met培养基中支持细胞生长的能力。这三个细胞系代谢l-同型半胱氨酸和5 ' -脱氧-5 ' -甲基硫代腺苷为蛋氨酸、s -腺苷- l-蛋氨酸和蛋白质。然而，代谢和生长研究之间存在差异：Raji细胞可以在l -同型半胱氨酸和5 ' -脱氧-5 ' -甲基硫代腺苷上生长，BHK细胞可以在l -同型半胱氨酸上生长，但不能在5 ' -脱氧-5 ' -甲基硫代腺苷上生长，CCL 39细胞既不能在l -同型半胱氨酸上生长，也不能在5 ' -脱氧-5 ' -甲基硫代腺苷上生长。在CCL 39和Raji细胞中，用25 μM[甲基-14c]蛋氨酸+ 25 μM[甲基-14c] 5′-脱氧-5′-甲基硫腺苷+ 25 μM蛋氨酸培养，研究了外源蛋氨酸和由5′-脱氧-5′-甲基硫腺苷内源性合成的蛋氨酸的代谢，两种细胞对外源蛋氨酸和内源性蛋氨酸的代谢均无差异。我们的结果表明i)“蛋氨酸依赖”最初描述为l -同型半胱氨酸[Hoffman， R.M.和Erbe， R.W. (1976) Proc. Natl。学会科学。USA 73,1523]，也可以与蛋氨酸的其他前体，即5 ' -脱氧-5 ' -甲基硫腺苷观察到；Ii)“蛋氨酸依赖”不能被认为是细胞不能依靠由前体内源性合成的蛋氨酸生长，而是取决于所使用的前体，并且可能取决于在没有蛋氨酸的情况下前体的毒性作用。
The metabolism of the two methionine precursors, L-homocysteine and 5′-deoxy-5′-methylthioadenosine was compared to the ability of these compounds to support cell growth in a Met-free medium, in the following mammalian cell lines : Raji, CCL 39 and BHK cells. These three cell lines metabolized L-homocysteine and 5′-deoxy-5′-methylthioadenosine into methionine, S-adenosyl-L-methionine and proteins. However there was a discrepancy between metabolic and growth studies : Raji cells could grow on L-homocysteine and on 5′-deoxy-5′-methylthioadenosine, BHK cells could grow on L-homocysteine but not on 5′-deoxy-5′-methylthioadenosine, and CCL 39 cells could not grow either on L-homocysteine or on 5′-deoxy-5′-methylthioadenosine. The metabolism of exogenous methionine, and of methionine endogenously synthesized from 5′-deoxy-5′-methylthioadenosine was studied in CCL 39 and Raji cells, incubated with 25 μM [methyl-14c] methionine + 25 μM 5′-deoxy-5′-methylthioadenosine or 25 μM [methyl-14c] 5′-deoxy-5′-methylthioadenosine + 25 μM methionine : there was no difference between the metabolism of exogenous and endogenous methionine in either type of cell. Our results indicate that i) “methionine dependence” initially described for L-homocysteine [Hoffman, R.M. and Erbe, R.W. (1976) Proc. Natl. Acad. Sci. USA 73, 1523], can also be observed with the other precursor of methionine, ie 5′-deoxy-5′-methylthioadenosine; ii) “methionine-dependence” can not be considered as the inability of a cell to grow on methionine endogenously synthesized from a precursor, but depends on the precursor used, and possibly, on a toxic effect of the precursor in the absence of methionine.
哺乳动物细胞中蛋氨酸的代谢和 5-甲硫腺苷和 5-甲硫肌苷的生长刺激。
DOI：
10.1016/0006-291x(83)91476-6
发表时间：
1983
影响因子：
3.1
作者：
Carson,DA;Willis,EH;Kamatani,N
通讯作者：
Kamatani,N