Severity of experimentally reactivated herpetic eye disease is related to the neurovirulence of the latent virus.
Severity of experimentally reactivated herpetic eye disease is related to the neurovirulence of the latent virus.
复制标题
实验性再激活的疱疹性眼病的严重程度与潜伏病毒的神经毒力有关。
DOI：
--
复制
发表时间：
1987
影响因子：
4.4
通讯作者：
D. Schaefer
中科院分区：
文献类型：
--
作者：
W. G. Stroop;D. Schaefer
作者研究了兔鼻内接种单纯疱疹病毒1型（HSV-1）高、低神经毒力株后急性和实验性再激活感染期间产生的眼病。潜伏性三叉神经节感染的实验性再激活是通过注射环磷酰胺，然后在第二天注射地塞米松来完成的。当单次注射时，两种药物都不能重新激活潜伏的HSV-1感染。在高神经毒力HSV-1株感染的急性期和再活化期，许多家兔发生非常严重的结膜炎和角膜炎。一些兔子出现了出血性角膜病变，还有一些失明。只有少数急性和再激活的弱神经毒力病毒株感染的家兔发生轻度结膜炎。高神经毒力菌株在再活化感染期间从泪液膜中回收的频率高于低神经毒力菌株。通过使用从纯化的病毒制备的3 H-标记的DNA探针三叉神经节组织原位，发现两种病毒株建立神经节潜伏期到大约相同的程度。再激活与每个神经节神经元的HSV-1 RNA量的增加和亚细胞位置的变化相关。这些研究表明，感染菌株的相对神经毒力决定了它从潜伏期重新激活的难易程度和所产生的重新激活的眼病的严重程度。
The authors examined the eye diseases produced during acute and experimentally reactivated infections of rabbits intranasally inoculated with high and low neurovirulent strains of herpes simplex virus, type-1 (HSV-1). Experimental reactivation of latent trigeminal ganglionic infection was accomplished by an injection of cyclophosphamide followed by one injection of dexamethasone the next day. Neither drug, when given as a single injection, reactivated latent HSV-1 infection. During acute and reactivated phases of high neurovirulent HSV-1 strain infection, many rabbits developed very severe conjunctivitis and keratitis. Some rabbits developed hemorrhagic corneal lesions, and a few became blind. Only a few rabbits with acute and reactivated low neurovirulent virus strain infections developed mild conjunctivitis. The high neurovirulent strain was recovered from tear film more frequently than the low neurovirulent strain during reactivated infections. By use of 3H-labelled DNA prepared from purified virus to probe trigeminal ganglionic tissues in situ, both strains of virus were found to establish ganglionic latency to about the same degree. Reactivation correlated with an increase in the amount of HSV-1 RNA per ganglionic neuron and a change in subcellular location. These studies indicate that the relative neurovirulence of the infecting strain determines the ease with which it can be reactivated from latency and the severity of the reactivated ocular disease produced.
登录
查看更多内容
单纯疱疹病毒 1 型基因功能的物理位置与 HSV 神经毒力增加 1000 万倍特别相关。
影响因子：
3.7
作者：
Thompson,RL;Wagner,EK;Stevens,JG
通讯作者：
Stevens,JG
人干扰素αA或αD和三氟尿苷治疗兔疱疹性角膜炎。
影响因子：
4.4
作者：
Trousdale,MD;Nesburn,AB
通讯作者：
Nesburn,AB
兔子实验性疱疹病毒角膜炎：局部感染途径与基质内感染途径。
影响因子：
2.1
作者：
Meyers-Elliott,RH;Chitjian,PA;Dethlefs,BA
通讯作者：
Dethlefs,BA
通过原位杂交在急性和潜伏感染期间将单纯疱疹病毒定位在小鼠中枢神经系统的三叉神经和嗅觉系统中。
DOI：
--
发表时间：
1984
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Stroop,WG;Rock,DL;Fraser,NW
通讯作者：
Fraser,NW
复发性疱疹性角膜炎患者分离株中病毒 DNA 的分析。
影响因子：
4.4
作者：
Asbell,PA;Centifanto-Fitzgerald,YM;Chandler,JW;Kaufman,HE
通讯作者：
Kaufman,HE