Follicle dynamics: visualization and analysis of follicle growth and maturation using murine ovarian tissue culture.
Follicle dynamics: visualization and analysis of follicle growth and maturation using murine ovarian tissue culture.
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DOI：
10.1007/s10815-017-1073-5
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发表时间：
2018-03
影响因子：
3.1
通讯作者：
Kikkawa F
中科院分区：
文献类型：
--
作者：
Murase T;Iwase A;Komatsu K;Bayasula;Nakamura T;Osuka S;Takikawa S;Goto M;Kotani T;Kikkawa F
利用生理浓度的卵泡刺激素(FSH)和黄体生成素(LH)对卵巢组织培养中的卵泡发育进行可视化和分析，以建立一种能够使卵泡在体外高效生长的卵巢组织培养体系。取4周龄雌性ICR小鼠卵巢组织进行切片培养。在显微镜下每隔24小时或30分钟获取培养中的卵巢组织的图像。追踪并分析卵巢组织切片中观察到的每个卵泡的面积与卵母细胞成熟的关系。使用这种培养系统，我们能够跟踪每个卵泡的发育。卵泡生长与卵母细胞成熟有关。33%的卵泡获得了成熟卵母细胞(MII)。大约四分之一的卵泡(24%)没有生长并导致闭锁。在小鼠卵巢组织培养中，成功地显示和分析了卵泡动力学。我们能够在体外从完全生长的卵泡中获得成熟的卵母细胞。该培养体系为卵巢组织的高效体外培养奠定了基础。本文的在线版本(10.1007/s10815-0171073-5)包含补充材料，可供授权用户使用。
To visualize and analyze follicle development in ovarian tissue culture using physiological concentrations of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in order to establish an ovarian tissue culture system that enables efficient in vitro growth of follicles. Ovarian tissues from 4-week-old female ICR mice were sliced and cultured. Images of ovarian tissues in culture were obtained at 24-h or 30-min intervals by using a microscope. The area of each follicle observed in the ovarian tissue slices was tracked and analyzed in association with oocyte maturation. We were able to track the development of each follicle using this culture system. Follicle growth was associated with oocyte maturation. Meiotically matured oocytes (MII) were obtained from 33% of all follicles investigated. Approximately, a quarter of follicles (24%) did not grow and resulted in atresia. Follicle dynamics were successfully visualized and analyzed in murine ovarian tissue culture. We were able to obtain mature oocytes from the fully grown follicles in vitro. This culture system would be helpful for efficient in vitro culturing of ovarian tissues. The online version of this article (10.1007/s10815-017-1073-5) contains supplementary material, which is available to authorized users.
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