Structural changes in chromosomes driven by multiple condensin motors during mitosis
Structural changes in chromosomes driven by multiple condensin motors during mitosis
复制标题
有丝分裂过程中由多个凝缩蛋白马达驱动的染色体结构变化
DOI：
10.1016/j.celrep.2023.112348
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发表时间：
2023
期刊：
影响因子：
8.8
通讯作者：
Thirumalai, D.
中科院分区：
文献类型：
--
作者：
Dey, Atreya;Shi, Guang;Takaki, Ryota;Thirumalai, D.
我们创建了一个计算框架，利用多个凝聚素I/II马达的环挤出（LE）来预测有丝分裂过程中染色体组织的变化。该理论准确地再现了HeLa和DT 40细胞中有丝分裂染色体的实验接触概率分布。LE率在有丝分裂开始时较小，并随着细胞接近中期而增加。缩合蛋白II介导的平均环大小比缩合蛋白I的环大约6倍。相互重叠的环被钉在LE过程期间由马达形成的中心动态变化的螺旋支架上。使用Hi-C接触图作为唯一输入的基于聚合物物理学的数据驱动方法表明，螺旋的特征在于随机螺旋变态（RHP），其中手性沿支架随机沿着变化。理论预测，这是可测试的成像实验，不包含任何参数。
We create a computational framework that utilizes loop extrusion (LE) by multiple condensin I/II motors to predict changes in chromosome organization during mitosis. The theory accurately reproduces the experimental contact probability profiles for the mitotic chromosomes in HeLa and DT40 cells. The LE rate is smaller at the start of mitosis and increases as the cells approach metaphase. Condensin II-mediated mean loop size is about six times larger than loops because of condensin I. The loops, which overlap each other, are stapled to a central dynamically changing helical scaffold formed by the motors during the LE process. A polymer physics-based data-driven method that uses the Hi-C contact map as the only input shows that the helix is characterized as random helix perversions (RHPs) in which the handedness changes randomly along the scaffold. The theoretical predictions, which are testable using imaging experiments, do not contain any parameters.
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影响因子：
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通讯作者：
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