Evaluation of ion mobility-mass spectrometry for determining the isomeric heterogeneity of oligosaccharide-alditols derived from bovine submaxillary mucin.
Evaluation of ion mobility-mass spectrometry for determining the isomeric heterogeneity of oligosaccharide-alditols derived from bovine submaxillary mucin.
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DOI：
10.1016/j.ijms.2013.07.015
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发表时间：
2013-10-15
影响因子：
1.8
通讯作者：
Hill HH Jr
中科院分区：
文献类型：
--
作者：
Li H;Bendiak B;Kaplan K;Davis E;Siems WF;Hill HH Jr
在糖组学研究中，碳水化合物的结构表征需要快速分离和异构体的独立分析。采用离子迁移率-质谱法分析了牛颌下腺粘蛋白中一系列中性寡糖-糖醇的异构体。采用了以下几种分析技术：(1)高效液相色谱离线分离低聚糖-糖醇混合物；(2)电喷雾电离-离子迁移率-飞行时间质谱法直接快速评价低聚糖的异构体异构性；(3)选择迁移率较高的MS2和MS3用双门离子迁移率-串联质谱法评价低聚糖的异构体迁移峰。在LC分离后的毫秒时间尺度上，观察到大多数低聚糖-糖醇的多个异构体离子迁移峰。使用双栅离子迁移率四极离子陷阱质谱仪，从同分异构体前体离子的碰撞诱导解离获得的碎裂光谱对于给定的前体m/z可能基本上相同，也可能截然不同。这进一步证实了在对同分异构体前体物种进行串联质谱分析之前，需要对其进行快速的物理拆分。
Rapid separation and independent analysis of isomeric species are needed for the structural characterization of carbohydrates in glycomics research. Ion mobility-mass spectrometry techniques were used to examine a series of isomeric neutral oligosaccharide-alditols derived from bovine submaxillary mucin. Several analytical techniques were employed: (1) off line separation of the oligosaccharide-alditol mixture by HPLC; (2) direct and rapid evaluation of isomeric heterogeneity of oligosaccharides by electrospray ionization-ion mobility-time of flight mass spectrometry; and (3) mobility-selected MS2 and MS3 to evaluate isomeric mobility peaks by dual gate ion mobility-tandem mass spectrometry. Multiple isomeric ion mobility peaks were observed for the majority of oligosaccharide-alditols, which was achieved on the millisecond time scale after LC separation. Fragmentation spectra obtained from the collision-induced dissociation of isomeric precursor ions could be essentially identical, or dramatically different for a given precursor m/z using the dual-gate ion mobility quadrupole ion trap mass spectrometer. This further confirmed the need for rapid physical resolution of isomeric precursor species prior to their tandem mass spectral analysis.
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影响因子：
2.2
作者：
Armstrong, GS;Mandelshtam, VA;Bendiak, B
通讯作者：
Bendiak, B
影响因子：
2.7
作者：
Jones, DNM;Bendiak, B
通讯作者：
Bendiak, B
影响因子：
1.8
作者：
McLean, JA;Ruotolo, BT;Russell, DH
通讯作者：
Russell, DH
影响因子：
3.3
作者：
Fenn, Larissa S.;McLean, John A.
通讯作者：
McLean, John A.
影响因子：
2.3
作者：
Clowers, BH;Hill, HH
通讯作者：
Hill, HH