The wis1 signal transduction pathway is required for expression of cAMP-repressed genes in fission yeast.
The wis1 signal transduction pathway is required for expression of cAMP-repressed genes in fission yeast.
复制标题
wis1 信号转导途径是裂殖酵母中 cAMP 抑制基因表达所必需的。
作者：
Sophie Stettler;E. Warbrick;Simon Prochnik;Shaun Mackie;P. Fantes
粟酒裂殖酵母的wis 1蛋白激酶是MAP激酶家族的成员。以前曾报道wis 1功能的丧失会导致G2有丝分裂转换延迟、稳定期活力丧失和对渗透压休克的超敏反应。它至少部分通过激活MAP激酶同源物sty 1发挥作用; sty 1功能丧失突变体与wis 1缺失突变体共享许多表型。我们在这里表明，此外，损失的wis 1功能导致有缺陷的接合，并抑制超接合表型的pat 1 -114突变。与此相一致的是，mei 2基因的诱导在wis 1突变体中是有缺陷的，而mei 2基因通常是由氮饥饿诱导的。在野生型细胞中，氮饥饿通过细胞内环AMP（cAMP）水平和cAMP依赖性蛋白激酶活性的下降导致mei 2诱导。我们在这里表明，wis 1功能所需的mei 2诱导氮饥饿。fbp 1基因的表达受cAMP负调控，以响应葡萄糖限制：fbp 1的诱导也需要wis 1和sty 1功能。wis 1的缺失相对于由腺苷酸环化酶（git 2/pk 1）或cAMP依赖性蛋白激酶（pka 1）功能缺失引起的fbp 1表达增加是上位性的。这些观察结果可以用一个模型来解释，在这个模型中，PKA 1通路负调节WIS 1通路，或者这两个通路可能独立地作用于下游靶点。支持后一种解释，至少在调节细胞分裂方面，观察到cAMP依赖性蛋白激酶（cgs 1）的调节亚基的功能丧失导致wis 1+和wis 1 δ遗传背景下分裂时细胞长度适度增加。
The wis1 protein kinase of Schizosaccharomyces pombe is a member of the MAP kinase kinase family. Loss of wis1 function has previously been reported to lead to a delay in the G2-mitosis transition, loss of viability in stationary phase, and hypersensitivity to osmotic shock. It acts at least in part by activating the MAP kinase homologue sty1; loss-of-function sty1 mutants share many phenotypes with wis1 deletion mutants. We show here that, in addition, loss of wis1 function leads to defective conjugation, and to suppression of the hyperconjugation phenotype of the pat1-114 mutation. Consistent with this, the induction of the mei2 gene, which is normally induced by nitrogen starvation, is defective in wis1 mutants. In wild-type cells, nitrogen starvation leads to mei2 induction through a fall in intracellular cyclic AMP (cAMP) level and activity of the cAMP-dependent protein kinase. We show here that wis1 function is required for mei2 induction following nitrogen starvation. Expression of the fbp1 gene is negatively regulated by cAMP in response to glucose limitation: induction of fbp1 also requires wis1 and sty1 function. Loss of wis1 is epistatic over increased fbp1 expression brought about by loss of adenylate cyclase (git2/cyr1) or cAMP-dependent protein kinase (pka1) function. These observations can be explained by a model in which the pka1 pathway negatively regulates the wis1 pathway, or the two pathways might act independently on downstream targets. The latter explanation is supported, at least as regards regulation of cell division, by the observation that loss of function of the regulatory subunit of the cAMP-dependent protein kinase (cgs1) brings about a modest increase in cell length at division in both wis1+ and wis1 delta genetic backgrounds.
粟酒裂殖酵母 pyp1 蛋白酪氨酸磷酸酶负向调节营养监测途径。
影响因子：
4
作者：
Santo,PaolaDal;Blanchard,Barbara;Hoffman,CharlesS
通讯作者：
Hoffman,CharlesS
影响因子：
4
作者：
S. Byrne;C. S. Hoffman
通讯作者：
S. Byrne;C. S. Hoffman
sck1 是裂殖酵母中 cAMP 依赖性蛋白激酶途径缺陷的高拷贝数抑制因子，编码与酿酒酵母 SCH9 激酶同源的蛋白质。
影响因子：
3.3
作者：
Jin,M;Fujita,M;Culley,BM;Apolinario,E;Yamamoto,M;Maundrell,K;Hoffman,CS
通讯作者：
Hoffman,CS