Enterorhabdus caecimuris sp. nov., a member of the family Coriobacteriaceae isolated from a mouse model of spontaneous colitis, and emended description of the genus Enterorhabdus Clavel et al. 2009.

Enterorhabdus caecimuris sp. nov., a member of the family Coriobacteriaceae isolated from a mouse model of spontaneous colitis, and emended description of the genus Enterorhabdus Clavel et al. 2009.
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DOI:
10.1099/ijs.0.015016-0
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发表时间:
2010-07
影响因子:
2.8
通讯作者:
Haller D
Haller D
中科院分区:
生物学3区
文献类型:
--
作者:
Clavel T;Duck W;Charrier C;Wenning M;Elson C;Haller D

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C3 H/HeJBir小鼠肠道炎症模型用于从盲肠悬浮液中分离革兰氏阳性、杆状、非孢子形成细菌(B7 T)。根据16 SrRNA基因部分序列分析,菌株B7 T属于Coriobacteriaceae放线菌纲,与Enterorhabdus mucosicola Mt 1B 8 T亲缘关系最近(97.6%)。 菌株B7 T的主要脂肪酸为C16:0(19.1%),呼吸醌类为单甲基化和二甲基化。   细胞耐氧,但只能在缺氧条件下生长。菌株B7 T不转化大豆苷元,对头孢噻肟耐药。通过DNA-DNA杂交试验和生理生化试验,将B7 T菌株与E.粘膜生的因此,菌株B7 T代表一个新的物种,建议将其命名为Enterorhabdus caecimuris sp. nov.。模式菌株为B7 T(=DSM 21839 T =CCUG 56815 T)。
The C3H/HeJBir mouse model of intestinal inflammation was used for isolation of a Gram-positive, rod-shaped, non-spore-forming bacterium (B7T) from caecal suspensions. On the basis of partial 16S rRNA gene sequence analysis, strain B7T was a member of the class Actinobacteria, family Coriobacteriaceae, and was related closely to Enterorhabdus mucosicola Mt1B8T (97.6 %). The major fatty acid of strain B7T was C16 : 0 (19.1 %) and the respiratory quinones were mono- and dimethylated. Cells were aerotolerant, but grew only under anoxic conditions. Strain B7T did not convert the isoflavone daidzein and was resistant to cefotaxime. The results of DNA–DNA hybridization experiments and additional physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain B7T from the type strain of E. mucosicola. Therefore, strain B7T represents a novel species, for which the name Enterorhabdus caecimuris sp. nov. is proposed. The type strain is B7T (=DSM 21839T =CCUG 56815T).
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