Srs2 prevents Rad51 filament formation by repetitive motion on DNA.
Srs2 prevents Rad51 filament formation by repetitive motion on DNA.
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DOI：
10.1038/ncomms3281
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发表时间：
2013
影响因子：
16.6
通讯作者：
Myong, Sua
中科院分区：
文献类型：
--
作者：
Qiu, Yupeng;Antony, Edwin;Doganay, Sultan;Koh, Hye Ran;Lohman, Timothy M.;Myong, Sua
Srs2 dismantles presynaptic Rad51 filaments and prevents its re-formation as an anti-recombinase. However, the molecular mechanism by which Srs2 accomplishes these tasks remains unclear. Here we report a single molecule fluorescence study of the dynamics of Rad51 filament formation and its disruption by Srs2. Rad51 forms filaments on ssDNA by sequential binding of primarily monomers and dimers in a 5’ to 3’ direction. One Rad51 molecule binds to 3 nucleotides and six monomers are required to achieve a stable nucleation cluster. Srs2 exhibits ATP-dependent repetitive scrunching of ssDNA and this activity prevents reformation of the Rad51 filament. The same activity of Srs2 cannot prevent RecA filament formation, indicating its specificity for Rad51. Srs2’s DNA unwinding activity is greatly suppressed when Rad51 filaments form on duplex DNA. Taken together, our results reveal an exquisite and highly specific mechanism by which Srs2 regulates Rad51 filament formation.
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