Vascular smooth muscle contractility depends on cell shape.
Vascular smooth muscle contractility depends on cell shape.
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DOI：
10.1039/c1ib00061f
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发表时间：
2011-11
期刊：
影响因子：
--
通讯作者：
Parker KK
中科院分区：
文献类型：
--
作者：
Alford PW;Nesmith AP;Seywerd JN;Grosberg A;Parker KK
平滑肌结构在确定动脉功能中的生理作用知之甚少。我们的目的是阐明血管平滑肌结构和功能性收缩输出之间的关系。使用微接触印刷和肌肉薄膜技术，我们设计了具有严格定义的几何形状的体外血管组织，并测试了它们的收缩功能。在所有组织中，血管平滑肌细胞（VSMCs）与体内样纺锤体结构高度一致，并在生理上收缩以响应内皮素-1的刺激。然而，组织中的VSMC被迫夸张的纺锤体伸长施加显着更大的收缩力，每单位横截面积比那些具有较小的纵横比。此外，这种增加的收缩并没有与传统测量的收缩表型标记物的增加一起发生。这些结果表明，血管组织内的细胞结构在赋予组织功能方面起着重要作用，并且在某些系统中，传统的表型表征不足以定义VSMC的功能性收缩群体。血管平滑肌功能在体外测量，并显示由细胞和组织结构决定
The physiologic role of smooth muscle structure in defining arterial function is poorly understood. We aimed to elucidate the relationship between vascular smooth muscle architecture and functional contractile output. Using microcontact printing and muscular thin film technology, we engineered in vitro vascular tissues with strictly defined geometries and tested their contractile function. In all tissues, vascular smooth muscle cells (VSMCs) were highly aligned with in vivo-like spindle architecture, and contracted physiologically in response to stimulation with endothelin-1. However, tissues wherein the VSMCs were forced into exaggerated spindle elongation exerted significantly greater contraction force per unit cross-sectional area than those with smaller aspect ratios. Moreover, this increased contraction did not occur in conjunction with an increase in traditionally measured contractile phenotype markers. These results suggest that cellular architecture within vascular tissues plays a significant role in conferring tissue function and that, in some systems, traditional phenotype characterization is not sufficient to define a functionally contractile population of VSMCs. Vascular smooth muscle function is measured in vitro and shown to be determined by cellulr and tissue architecture
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影响因子：
--
作者：
Bray, Mark-Anthony;Sheehy, Sean P.;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
影响因子：
46.9
作者：
Kim, BS;Nikolovski, J;Mooney, DJ
通讯作者：
Mooney, DJ
影响因子：
20.1
作者：
Ichii, T;Koyama, H;Nishizawa, Y
通讯作者：
Nishizawa, Y
影响因子：
56.9
作者：
MECHAM, RP;WHITEHOUSE, LA;VOELKEL, NF
通讯作者：
VOELKEL, NF
DOI：
10.1073/pnas.94.3.849
发表时间：
1997-02-04
影响因子：
11.1
作者：
Maniotis, AJ;Chen, CS;Ingber, DE
通讯作者：
Ingber, DE