Regulation of Terminal Differentiation of Cultured Mouse Epidermal Cells by lα,25-Dihydroxyvitamin D3
Regulation of Terminal Differentiation of Cultured Mouse Epidermal Cells by lα,25-Dihydroxyvitamin D3
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lα,25-二羟基维生素 D3 对培养小鼠表皮细胞终末分化的调节
DOI：
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
T. Kuroki
中科院分区：
文献类型：
--
作者：
J. Hosomi;J. Hosoi;E. Abe;T. Suda;T. Kuroki
发现小鼠表皮细胞在原代培养中的终末分化受lα，25-二羟基维生素D3（lα，25（OH）2D 3）的调节，l α，25（OH）2D 3是维生素D3的激素形式，通过在肝脏和肾脏中连续羟基化产生。在0.12 nM或更高的浓度下，1 α，25（OH）2D 3剂量依赖性地刺激表皮分化。在维生素的存在下，分层病灶的数量和大小增加，相邻病灶合并。在处理的培养物中的基底细胞急剧减少，并经历分化成鳞状和去核细胞脱落到培养基中培养过程中。在分化过程中，细胞体积增大，密度降低。1 α，25（OH）2D 3显著刺激皮层包膜的形成，这是一种在质膜下形成的具有化学稳定交联的结构。在所检测的几种维生素D3衍生物中，1 α，25（OH）2D 3在诱导表皮分化方面是最有效的。刺激表皮...
Terminal differentiation of mouse epidermal cells in primary culture was found to be regulated by lα,25- dihydroxyvitamin D3 (lα,25(OH)2D3), the hormonal form of vitamin D3 produced by sequential hydroxylations in the liver and kidney. Epidermal differentiation was stimulated dose dependently by lα,25(OH)2D3 at concentrations of 0.12 nM or more. In the presence of the vitamin, stratified foci increased in number and size and contiguous foci coalesced. Basal cells in the treated cultures decreased sharply and underwent differentiation into squamous and enucleated cells which sloughed off into the medium during cultivation. The size and density of the cells became larger and lighter during the course of differentiation. lα,25(OH)2D3 markedly stimulated formation of a cornified envelope, a structure with chemically stable cross-links formed beneath the plasma membrane. Of several derivatives of vitamin D3 examined, lα,25(OH)2D3 was the most potent in inducing epidermal differentiation. Stimulation of epiderm...
大鼠皮肤中 1,25-二羟基维生素 D3 受体样蛋白的表征。
DOI：
10.1073/pnas.77.10.5822
发表时间：
1980
影响因子：
11.1
作者：
Simpson,RU;DeLuca,HF
通讯作者：
DeLuca,HF