Optical quantal analysis of synaptic transmission in wild-type and rab3-mutant Drosophila motor axons.
Optical quantal analysis of synaptic transmission in wild-type and rab3-mutant Drosophila motor axons.
复制标题
DOI：
10.1038/nn.2767
复制
发表时间：
2011-04
影响因子：
25
通讯作者：
Isacoff EY
中科院分区：
文献类型：
--
作者：
Peled ES;Isacoff EY
从神经元到其靶细胞的突触传递通过从数十到数千个突触前释放位点释放神经递质而发生，这些位点的强度和可塑性可以相当大地变化。我们报告了一种在体内成像方法，监测实时突触传递同时在许多释放网站的量子分辨率。我们将这种方法应用于模型果蝇幼虫神经肌肉接头的神经元能系统。我们发现，在基础条件下，约有一半的释放网站有一个非常低的释放概率，但这些穿插与网站高达50倍的概率。配对脉冲刺激抑制高概率位点，促进低概率位点，并招募先前沉默的位点。小的GTCRab 3的突变大大增加了释放概率，但仍然留下大约一半的位点沉默。我们的研究结果表明，基础突触强度和短期可塑性的调节在个别网站的释放概率的水平。
Synaptic transmission from a neuron to its target cells occurs via neurotransmitter release from dozens to thousands of presynaptic release sites whose strength and plasticity can vary considerably. We report an in vivo imaging method that monitors real-time synaptic transmission simultaneously at many release sites with quantal resolution. We applied this method to the model glutamatergic system of the Drosophila melanogaster larval neuromuscular junction. We find that, under basal conditions, about half of release sites have a very low release probability, but these are interspersed with sites with as much as a 50-fold higher probability. Paired-pulse stimulation depresses high-probability sites, facilitates low-probability sites, and recruits previously silent sites. Mutation of the small GTPase Rab3 substantially increases release probability but still leaves about half of the sites silent. Our findings suggest that basal synaptic strength and short-term plasticity are regulated at the level of release probability at individual sites.
登录
查看更多内容
影响因子：
56.9
作者：
BETZ, WJ;BEWICK, GS
通讯作者：
BEWICK, GS
影响因子：
25
作者：
Oertner, TG;Sabatini, BL;Svoboda, K
通讯作者：
Svoboda, K
影响因子：
25
作者：
Guerrero, G;Reiff, DF;Isacoff, EY
通讯作者：
Isacoff, EY
DOI：
10.1073/pnas.91.22.10380
发表时间：
1994-10-25
影响因子：
11.1
作者：
ALLEN, C;STEVENS, CF
通讯作者：
STEVENS, CF
影响因子：
7.8
作者：
Anderson, JC;Baddeley, RJ;Hanlon, RT
通讯作者：
Hanlon, RT