miR-335-5p suppresses gastric cancer progression by targeting MAPK10.
miR-335-5p suppresses gastric cancer progression by targeting MAPK10.
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DOI：
10.1186/s12935-020-01684-z
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发表时间：
2021-01-22
影响因子：
5.8
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Gao Y;Wang Y;Wang X;Zhao C;Wang F;Du J;Zhang H;Shi H;Feng Y;Li D;Yan J;Yao Y;Hu W;Ding R;Zhang M;Wang L;Huang C;Zhang J
最近的研究已经确定了microRNAs(MiRNAs)在癌症进展中的作用。MiR-335-5p在包括胃癌在内的多种肿瘤中的异常表达已有报道。本研究评估了miR-335-5p在GC中的确切作用以及其作用的分子机制，包括其靶标MAPK10的作用。用实时定量聚合酶链式反应检测胃癌细胞系和组织中miR-335-5p的表达水平。用四甲基偶氮唑盐比色法和集落形成实验检测细胞增殖，用Transwell和伤口愈合实验检测GC细胞的侵袭和迁移。分析胃癌miR-335-5p表达水平与细胞周期相关靶基因丝裂原活化蛋白激酶10(MAPK10)的相关性。此外，通过荧光素酶报告实验、qRT-PCR和Western blotting对候选靶标进行了评估。MiR-335-5p在胃癌组织和细胞系中表达下调。此外，miR-335-5P还可抑制GC细胞的增殖和迁移，并诱导其凋亡。此外，在体外，miR-335-5p使胃癌细胞周期停滞在G1/S期。MiR-335-5p与细胞周期相关靶基因MAPK10在胃癌中的表达水平相关，miR-335-5p直接靶向MAPK10。这些数据表明miR-335-5p是一种肿瘤抑制因子，并通过MAPK10抑制GC进展。
Recent studies have established the roles of microRNAs (miRNAs) in cancer progression. The aberrant expression of miR-335-5p has been reported in many cancers, including gastric cancer (GC). In this study, the precise roles of miR-335-5p in GC as well as the molecular mechanisms underlying its effects, including the role of its target MAPK10, were evaluated. Quantitative real-time PCR was used to evaluate miR-335-5p levels in GC cell lines and tissues. MTT and colony formation assays were used to detect cell proliferation, and Transwell and wound-healing assays were used to evaluate the invasion and migration of GC cells. The correlation between levels of miR-335-5p and the cell cycle-related target gene mitogen-activated protein kinase 10 (MAPK10) in GC was analyzed. In addition, the candidate target was evaluated by a luciferase reporter assay, qRT-PCR, and western blotting. The levels of miR-335-5p were downregulated in GC tissues and cell lines. Furthermore, miR-335-5p inhibited the proliferation and migration of GC cells and induced apoptosis. Additionally, miR-335-5p arrested the cell cycle at the G1/S phase in GC cells in vitro. Levels of miR-335-5p and the cell cycle-related target gene MAPK10 in GC were correlated, and MAPK10 was directly targeted by miR-335-5p. These data suggest that miR-335-5p is a tumor suppressor and acts via MAPK10 to inhibit GC progression.
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影响因子：
6.4
作者：
Slattery, Martha L.;Herrick, Jennifer S.;Mullany, Lila E.;Valeri, Nicola;Stevens, John;Caan, Bette J.;Samowitz, Wade;Wolff, Roger K.
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通讯作者：
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DOI：
10.26355/eurrev_201907_18429
发表时间：
2019-07-01
影响因子：
3.3
作者：
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影响因子：
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作者：
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影响因子：
11.2
作者：
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通讯作者：
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