Utilizing Paper-Based Devices for Antimicrobial-Resistant Bacteria Detection.
Utilizing Paper-Based Devices for Antimicrobial-Resistant Bacteria Detection.
复制标题
利用基于纸张的设备进行抗菌抗性细菌检测。
DOI：
10.1002/anie.201702776
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发表时间：
2017-06-06
期刊：
影响因子：
--
通讯作者：
Henry CS
中科院分区：
文献类型：
--
作者：
Boehle KE;Gilliand J;Wheeldon CR;Holder A;Adkins JA;Geiss BJ;Ryan EP;Henry CS
由于抗菌剂的广泛使用，抗菌素耐药性(AMR)，即细菌对抗菌药物作用的抵抗能力一直在上升。根据世界卫生组织的数据，AMR在美国的年成本估计为340亿美元，预计到2050年将成为全球头号死因。AMR细菌传播的一种方式，以及个人可能感染AMR感染的地方，是通过受污染的水。监测环境AMR细菌目前需要将样本运送到中心实验室进行缓慢和劳动密集型测试。我们已经开发了一种使用纸基分析设备(PADS)的廉价检测方法，可以测试β-内酰胺酶介导的耐药性作为AMR的一种形式。为了证明其可行性，使用PAD检测了废水和污水中β-内酰胺类抗生素的耐药性，并对从环境水源中分离的单个细菌进行了耐药性鉴定。由于抗菌剂的广泛使用，抗菌素耐药性(AMR)，即细菌物种抵抗抗菌药作用的能力一直在上升，AMR传播的许多方式之一是通过受污染的水源。为了监测这些水源，我们开发了一种使用纸基分析设备(PAD)的廉价、快速的检测方法，该设备可以测试β-内酰胺酶介导的耐药性的存在，作为AMR的一种主要形式，可以可靠地检测污水中的耐药性。
Antimicrobial resistance (AMR), the ability of a bacterial species to resist the action of an antimicrobial drug, has been on the rise due to the widespread use of antimicrobial agents. Per the World Health Organization, AMR has an estimated annual cost of $34B in the US, and is predicted to be the number one cause of death worldwide by 2050. One way AMR bacteria can spread, and where individuals can contract AMR infections, is through contaminated water. Monitoring environment AMR bacteria currently requires samples be transported to a central laboratory for slow and labor intensive tests. We have developed an inexpensive assay using paper-based analytical devices (PADs) that can test for the presence of β-lactamase-mediated resistance as a form of AMR. To demonstrate viability, the PAD was used to detect β-lactam resistance in wastewater and sewage, and identified resistance in individual bacteria species isolated from environmental water sources. Antimicrobial resistance (AMR), the ability of a bacterial species to resist the action of an antimicrobial drug, has been on the rise due to the widespread use of antimicrobial agents, and one of the many ways AMR can spread is through contaminated water sources. To monitor these water sources, we have developed an inexpensive, fast assay using a paper-based analytical device (PAD) that can test for the presence of β-lactamase-mediated resistance as one major form of AMR that has reliably detected resistance in sewage water.
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