Integrating Sugar Metabolism With Transport: Elevation of Endogenous Cell Wall Invertase Activity Up-Regulates SlHT2 and SlSWEET12c Expression for Early Fruit Development in Tomato.
Integrating Sugar Metabolism With Transport: Elevation of Endogenous Cell Wall Invertase Activity Up-Regulates SlHT2 and SlSWEET12c Expression for Early Fruit Development in Tomato.
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将糖代谢与运输结合起来：内源细胞壁转化酶活性的升高上调SlHT2和SlSWEET12c表达，促进番茄早期果实发育
DOI：
10.3389/fgene.2020.592596
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发表时间：
2020
影响因子：
3.7
通讯作者：
Ruan YL
中科院分区：
文献类型：
--
作者：
Ru L;He Y;Zhu Z;Patrick JW;Ruan YL
早期果实发育对决定作物产量至关重要。细胞壁转化酶（CWIN）和糖转运蛋白在碳分配和植物发育中都起着重要作用。然而，关于果实发育过程中CWIN与糖转运蛋白之间关系的研究却很少。通过利用CWIN活性升高的转基因番茄，我们研究了CWIN活性的增加如何调节果实发育过程中糖转运基因的表达。我们的分析表明，CWIN活性可能受到多种调控因子的严格调控，包括开花前番茄子房中的两种转化酶抑制剂（INVINHs）和一种缺陷CWIN （deCWIN）。在糖转运体中，CWIN活性的升高分别在番茄开花后10天和15天增强了蔗糖外排SlSWEET12c和己糖摄取SlHT2的表达。结果表明，当韧皮部的蔗糖外排和薄壁细胞对己糖的摄取旺盛时，一些特定的糖最终会被输出转运体（SWEETs）和己糖转运体（HTs）响应以提高CWIN活性，可能是为了促进果实的快速膨胀。这些分析为通过操纵CWIN响应糖转运蛋白和CWIN本身来促进果实发育和糖积累提供了新的线索。
Early fruit development is critical for determining crop yield. Cell wall invertase (CWIN) and sugar transporters both play important roles in carbon allocation and plant development. However, there is little information about the relationship between CWIN and those functionally related sugar transporters during fruit development. By using transgenic tomato with an elevated CWIN activity, we investigated how an increase in CWIN activity may regulate the expression of sugar transporter genes during fruit development. Our analyses indicate that CWIN activity may be under tight regulation by multiple regulators, including two invertase inhibitors (INVINHs) and one defective CWIN (deCWIN) in tomato ovaries prior to anthesis. Among the sugar transporters, expression of SlSWEET12c for sucrose efflux and SlHT2 for hexose uptake was enhanced by the elevated CWIN activity at 10 and 15 days after anthesis of tomato fruit development, respectively. The findings show that some specific sugars will eventually be exported transporters (SWEETs) and hexose transporters (HTs) respond to elevate CWIN activity probably to promote rapid fruit expansion when sucrose efflux from phloem and hexose uptake by parenchyma cell are in high demand. The analyses provide new leads for improving crop yield by manipulating CWIN-responsive sugar transporters, together with CWIN itself, to enhance fruit development and sugar accumulation.
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影响因子：
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作者：
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