The program for processing newly synthesized histones H3.1 and H4.
The program for processing newly synthesized histones H3.1 and H4.
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DOI：
10.1038/nsmb.1911
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发表时间：
2010-11
影响因子：
16.8
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中科院分区：
文献类型：
--
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The mechanism by which newly synthesized histones are imported into the nucleus and deposited onto replicating chromatin alongside segregating nucleosomal counterparts is poorly understood, yet this program is expected to bear on the putative epigenetic nature of histone posttranslational modifications. In order to define the events by which naïve pre-deposition histones are imported into the nucleus, we biochemically purified and characterized the gamut of histone H3.1-containing complexes from human cytoplasmic fractions and identified their associated histone PTMs. Through reconstitution assays, biophysical analyses, and live cell manipulations, we describe in detail this series of events, namely the assembly of H3-H4 dimers, the acetylation of histones by the HAT1 holoenzyme, and the transfer of histones between chaperones that culminates with their karyopherin-mediated nuclear import. We further demonstrate the high degree of conservation for this pathway between higher and lower eukaryotes.
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影响因子：
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作者：
Natsume, Ryo;Eitoku, Masamitsu;Senda, Toshiya
通讯作者：
Senda, Toshiya
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Fillingham, Jeffrey;Recht, Judith;Greenblatt, Jack F.
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Greenblatt, Jack F.
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Groth, Anja
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Beavis, RC
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Alekseev, OM;Widgren, EE;O'Rand, MG
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O'Rand, MG