Codon-usage frequency mediated SNPs selection in lasR gene of cystic fibrosis Pseudomonas aeruginosa isolates.
Codon-usage frequency mediated SNPs selection in lasR gene of cystic fibrosis Pseudomonas aeruginosa isolates.
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密码子使用频率介导囊性纤维化铜绿假单胞菌 lasR 基因中的 SNP 选择。
DOI：
10.1016/j.micres.2019.05.002
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发表时间：
2019
影响因子：
6.7
通讯作者：
Weijun Dai
中科院分区：
文献类型：
--
作者：
Huifang Qiu;Yuanhao Li;Weijun Dai
铜绿假单胞菌是一种条件致病菌，在医院感染中具有很高的临床相关性。临床菌株DNA测序结果的积累。铜绿假单胞菌在lasR基因上发现了频繁的突变，该基因编码群体感应系统(QS)的最高ARES成分。我们分析了大量囊性纤维化铜绿假单胞菌flasR基因的测序数据。我们的系统分析表明，lasR基因的单核苷酸多态(SNPs)选择在很大程度上受到密码子使用频率的限制。总体而言，编码非保守氨基酸的SNP替代密码子导致密码子使用频率相对较低，而与保守氨基酸相关的密码子则没有受到严格的调控。这些SNPs替换导致了不同的功能LasR亚型，并有助于重组子RasR变异株的相对生长适合性。我们的调查揭示了一种新的Cf分离株lasR基因的SNPs选择模式。我们的研究结果可以为了解临床分离株在环境约束下的适应机制和开发针对CF患者的抗菌药物提供有力的资源。
Pseudomonas aeruginosais an opportunistic pathogen with high clinical relevance for hospital infections of patients. Accumulating DNA sequencing results of clinicalP. aeruginosaisolates have revealed frequent mutations inlasRgene, which encodes the highest arches component of quorum-sensing system (QS). We analyzed the sequencing data oflasRgene from a large collection of cystic fibrosis (CF)P. aeruginosaisolates. Our systematical analyses revealed that single nucleotide polymorphisms (SNPs) selection inlasRgene were largely constrained by codon-usage frequency. As a whole, SNP-substituted codons encoding unconserved amino acid resulted in unfavored codons with relatively low codon-usage frequency, while those associating with conserved amino acid were not strictly regulated in such way. These SNPs substitutions gives rise to diverse functional LasR isoforms and contributes to the relative growth fitness of recombinantlasRvariant strains. Our survey reveals a novel pattern of SNPs selections inlasRgene of CF isolates. Our findings could be served as a powerful resource for understanding adaptive mechanism of clinical isolates under environmental constrains and developing anti-bacteria drugs for CF patients.
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