Loss-of-function mutation of rice SLAC7 decreases chloroplast stability and induces a photoprotection mechanism in rice.

Loss-of-function mutation of rice SLAC7 decreases chloroplast stability and induces a photoprotection mechanism in rice.
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DOI:
10.1111/jipb.12350
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发表时间:
2015-12
影响因子:
11.4
通讯作者:
Lin Y
Lin Y
中科院分区:
生物学1区
文献类型:
--
作者:
Fan X;Wu J;Chen T;Tie W;Chen H;Zhou F;Lin Y

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植物吸收阳光来为光合作用的光化学反应提供动力,这可能会损坏光合作用机制。然而,保护叶绿体免受损害的机制仍不清楚。在这项工作中,我们证明了水稻 (Oryza sativa L.) SLAC7 是一种普遍表达的膜蛋白。在正常光照条件下,SLAC7 的功能丧失会导致突变叶片的叶绿体持续受损。 slac7-1 中与叶片损伤相关的离子泄漏指标(例如 H2O2 和脱落酸水平)显着高于野生型。一致地,slac7-1的光合作用效率和Fv/Fm比显着降低(类似于光抑制)。为了响应叶绿体损伤,slac7-1 通过植物激素和转录因子之间的协同作用改变其叶子形态(卷曲或融合的叶子),以减少光的吸收,这表明 slac7-1 中激活了叶绿体损伤的光保护机制。当在黑暗条件下生长时,slac7-1 显示出正常的表型。 AtSLAC1 启动子控制下的 SLAC7 可以部分补充拟南芥 slac1 突变体的表型,表明 SLAC 蛋白功能的部分保守。这些结果表明 SLAC7 对于维持水稻叶绿体的稳定性至关重要。
Plants absorb sunlight to power the photochemical reactions of photosynthesis, which can potentially damage the photosynthetic machinery. However, the mechanism that protects chloroplasts from the damage remains unclear. In this work, we demonstrated that rice (Oryza sativa L.) SLAC7 is a generally expressed membrane protein. Loss‐of‐function of SLAC7 caused continuous damage to the chloroplasts of mutant leaves under normal light conditions. Ion leakage indicators related to leaf damage such as H2O2 and abscisic acid levels were significantly higher in slac7‐1 than in the wild type. Consistently, the photosynthesis efficiency and Fv/Fm ratio of slac7‐1 were significantly decreased (similar to photoinhibition). In response to chloroplast damage, slac7‐1 altered its leaf morphology (curled or fused leaf) by the synergy between plant hormones and transcriptional factors to decrease the absorption of light, suggesting that a photoprotection mechanism for chloroplast damage was activated in slac7‐1. When grown in dark conditions, slac7‐1 displayed a normal phenotype. SLAC7 under the control of the AtSLAC1 promoter could partially complement the phenotypes of Arabidopsis slac1 mutants, indicating a partial conservation of SLAC protein functions. These results suggest that SLAC7 is essential for maintaining the chloroplast stability in rice.
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