Interaction between MdZAT10a-like and MdbHLH100 negatively regulates salt tolerance in apple (Malus domestica Borkh.)
Interaction between MdZAT10a-like and MdbHLH100 negatively regulates salt tolerance in apple (Malus domestica Borkh.)
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MdZAT10a-like 和 MdbHLH100 之间的相互作用对苹果 (Malus Domestica Borkh) 的耐盐性产生负调节。
DOI：
10.1016/j.envexpbot.2022.104938
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发表时间：
2022-08
影响因子：
5.7
通讯作者：
Nan Wang
中科院分区：
文献类型：
--
作者：
Lei Yu;Zhangwen Guo;Wenjun Liu;Xingyi Feng;Zhiqiang Li;Qi Zou;Hongcheng Fang;Zongying Zhang;Zhiquan Mao;Xuesen Chen;Zijing Chen;Nan Wang
盐分是一种有害的环境因子，它抑制植物生长，降低包括苹果在内的农作物的产量和品质。以野生型苹果“皇家嘎拉”组培苗为试材，用不同浓度的NaCl溶液进行处理。通过qRT-PCR分析筛选到一个拟南芥锌指（ZAT）转录因子，命名为MdZAT 10a-like，并显示其对盐胁迫有显著响应。MdZAT 10a-like基因在苹果愈伤组织中的过表达降低了MdNHX 1基因的表达，从而增强了愈伤组织对盐胁迫的敏感性。酵母双杂交筛选表明MdbHLH 100能够与MdZAT 10a-like相互作用。MdZAT 10a样-MdbHLH 100蛋白复合物显著抑制下游基因MdNHX 1的表达。目前的功能分析MdZAT 10a-like提供了有价值的见解苹果耐盐性的调控网络。·MdZAT 10a样的功能分析为盐耐受性提供了有价值的见解。·在苹果愈伤组织中过表达MdZAT 10a-like抑制其在盐胁迫下的生长。·MdZAT 10a样结合MdNHX 1的启动子以抑制其转录。·MdbHLH 100可通过形成蛋白复合物显著促进MdZAT 10a样对MdNHX 1表达的抑制作用。
Salinity is a harmful environmental factor that inhibits plant growth, and reduces the yield and quality of crops, including apple ( Malus ×domestica Borkh.). In this study, we treated tissue-cultured wild-type apple ‘Royal Gala’ seedlings with different concentrations of NaCl. A zinc finger of Arabidopsis thaliana (ZAT) transcription factor, designated MdZAT10a-like , was screened by qRT-PCR analysis and shown to be significantly responsive to salt stress. Overexpression of MdZAT10a-like in apple callus reduced the expression of MdNHX1 and thereby enhanced the sensitivity of callus to salt stress. Yeast two-hybrid screening showed that MdbHLH100 was capable of interacting with MdZAT10a-like. The MdZAT10a-like–MdbHLH100 protein complex significantly inhibited the expression of the downstream gene MdNHX1 . The present functional analyses of MdZAT10a-like provide valuable insights into the regulatory network of salt tolerance in apple. • Functional analyses of MdZAT10a-like provide valuable insights into the salt tolerance. • Overexpression of MdZAT10a-like in apple calli inhibits their growth under salt stress. • MdZAT10a-like binds to the promoter of MdNHX1 to inhibit its transcription. • MdbHLH100 can significantly promote the inhibitory effect of MdZAT10a-like on the expression of MdNHX1 by forming a protein complex.
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影响因子：
7.4
作者：
Sakamoto, H;Maruyama, K;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
DOI：
10.1016/j.agwat.2011.07.021
发表时间：
2011-11
期刊：
Fuel and Energy Abstracts
影响因子：
--
作者：
Z. Muyen;G. Moore;R. Wrigley
通讯作者：
Z. Muyen;G. Moore;R. Wrigley
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影响因子：
6.2
作者：
Bo, Chen;Chen, Haowei;Cai, Ronghao
通讯作者：
Cai, Ronghao
影响因子：
11.6
作者：
Davletova, S;Rizhsky, L;Mittler, R
通讯作者：
Mittler, R
影响因子：
6.2
作者：
X. Nguyen;S. H. Kim;Kyun Oh Lee;Kyung Eun Kim;Xiao-Min Liu;H. Han;M. Hoang;Shin-Woo Lee;
通讯作者：
X. Nguyen;S. H. Kim;Kyun Oh Lee;Kyung Eun Kim;Xiao-Min Liu;H. Han;M. Hoang;Shin-Woo Lee;