Functional Microarray Platform with Self-Assembled Monolayers on 3C-Silicon Carbide.
Functional Microarray Platform with Self-Assembled Monolayers on 3C-Silicon Carbide.
复制标题
在 3C-碳化硅上具有自组装单层的功能微阵列平台。
作者：
O. Cooper;Hoang‐Phuong Phan;Bei Wang;Sean E. Lowe;C. Day;N. Nguyen;J. Tiralongo
目前可用的生物平台，如微阵列和表面等离子体共振器不能将联合收割机高通量多路复用与无标记检测相结合。因此，新兴的微机电系统（MEMS）和微等离子体平台提供了生物和化学分析物的高分辨率、高通量无标记感测的潜力。因此，寻找能够结合多重和无标记定量的材料具有重要意义。最近，由于碳化硅（SiC）的化学惰性、机械强度、生物和血液相容性以及碳的存在，使得石墨烯的无转移生长成为可能，因此对碳化硅（SiC）作为许多生物医学应用中的合适材料的兴趣有所增加。SiC也是多功能的宽带隙半导体和有效的低损耗等离子体材料，因此是理想的用于增强生物传感器中的电流生物换能器。此外，立方变体3C-SiC是用于MEMS的非常有前途的材料，是用于微悬臂梁的容易微加工的合适平台，并且因此能够实现真实的时间小型化多路复用测定的潜力。因此，产生适当的功能化和通用的有机单层适合于生物分子的固定化是至关重要的，探索无标记的，多路复用的定量生物相互作用的SiC。在此，我们解决了使用各种硅烷自组装单分子膜（SAM）的共价功能化的单晶3C-SiC薄膜作为一种新的平台，用于功能化的微阵列表面的生成使用高通量聚糖阵列作为模型系统。我们还展示了在独立的SiC微结构上自动打印高通量阵列的能力。基于SiC的无标记聚糖阵列的实现将提供一个原理证明，该原理可以扩展到以类似的基于SiC的阵列格式固定其他生物分子，从而对研究生物相互作用的方式产生潜在的重大进展。
Currently available bioplatforms such as microarrays and surface plasmon resonators are unable to combine high-throughput multiplexing with label-free detection. As such, emerging microelectromechanical systems (MEMS) and microplasmonics platforms offer the potential for high-resolution, high-throughput label-free sensing of biological and chemical analytes. Therefore, the search for materials capable of combining multiplexing and label-free quantitation is of great significance. Recently, interest in silicon carbide (SiC) as a suitable material in numerous biomedical applications has increased due to its well-explored chemical inertness, mechanical strength, bio- and hemocompatibility, and the presence of carbon that enables the transfer-free growth of graphene. SiC is also multifunctional as both a wide-band-gap semiconductor and an efficient low-loss plasmonics material and thus is ideal for augmenting current biotransducers in biosensors. Additionally, the cubic variant, 3C-SiC, is an extremely promising material for MEMS, being a suitable platform for the easy micromachining of microcantilevers, and as such capable of realizing the potential of real time miniaturized multiplexed assays. The generation of an appropriately functionalized and versatile organic monolayer suitable for the immobilization of biomolecules is therefore critical to explore label-free, multiplexed quantitation of biological interactions on SiC. Herein, we address the use of various silane self-assembled monolayers (SAMs) for the covalent functionalization of monocrystalline 3C-SiC films as a novel platform for the generation of functionalized microarray surfaces using high-throughput glycan arrays as the model system. We also demonstrate the ability to robotically print high throughput arrays on free-standing SiC microstructures. The implementation of a SiC-based label-free glycan array will provide a proof of principle that could be extended to the immobilization of other biomolecules in a similar SiC-based array format, thus making potentially significant advances to the way biological interactions are studied.
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影响因子：
4.3
作者：
Tao, Sheng-Ce;Li, Yu;Schneck, Jonathan P.
通讯作者：
Schneck, Jonathan P.
影响因子：
7.4
作者：
Hansen, KM;Ji, HF;Thundat, T
通讯作者：
Thundat, T
影响因子：
4.4
作者：
Oyelaran, Oyindasola;McShane, Lisa M.;Dodd, Lori;Gildersleeve, Jeffrey C.
通讯作者：
Gildersleeve, Jeffrey C.
影响因子：
3.6
作者：
Huflejt, Margaret E.;Vuskovic, Marko;Bovin, Nicolai
通讯作者：
Bovin, Nicolai