Study on the inhibition of PLD on IAV-induced pulmonary macrophage based on autophagy and apoptosis
Study on the inhibition of PLD on IAV-induced pulmonary macrophage based on autophagy and apoptosis
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基于自噬和凋亡的PLD抑制IAV诱导的肺巨噬细胞的研究
DOI：
10.4103/pm.pm_207_19
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发表时间：
2020-01
影响因子：
0.7
通讯作者：
He Qing-Hu
中科院分区：
文献类型：
--
作者：
Li Ling;Ning Yi;Wei Ke;Hu Jue;Wu Tao;Zhao Cheng;Lu Fang-Guo;He Qing-Hu
背景资料：桔梗皂苷D（PlatycodinD，PLD）是桔梗干燥根中主要的三萜皂苷类化合物，在中医药中用于治疗呼吸系统疾病。本研究旨在评价PLD对甲型流感病毒（IAV）体外诱导的小鼠肺泡巨噬细胞的治疗作用，并探讨PLD的作用机制。材料和方法：Raw 264.7小鼠肺泡巨噬细胞在培养物中生长，细胞计数试剂盒-8测定细胞活力。用递增剂量的PLD（0、5、10、20、40、60、80、100 μM）处理细胞24 h。Annexin V-FITC/PI法和Tunel法检测细胞凋亡。采用酶联免疫吸附法检测细胞培养上清中白细胞介素（IL）-1 β、IL-6和肿瘤坏死因子（TNF）-α的水平。透射电镜观察吞噬细胞、自噬体、自溶酶体的数量及细胞超微结构。Western blotting检测微管相关蛋白轻链（LC）3 I、LC 3 II、Beclin-1和B细胞淋巴瘤（Bcl 2）的相对蛋白表达。用荧光显微镜观察Beclin-1和Bcl 2的定位。结果如下：PLD对Raw 264.7细胞的生长有明显的抑制作用，24 h的半数抑制浓度为115.74 μM，呈剂量依赖性。流感病毒感染Raw 264.7细胞后，PLD（2.5、5、10 μM）可提高细胞活性，减少细胞凋亡，降低细胞上清液中IL-1 β、IL-6、TNF-α的浓度，减轻细胞自噬损伤，调节Beclin-1、Bcl-2蛋白表达。结论：PLD可剂量依赖性地抑制IAV诱导的巨噬细胞过度凋亡和自噬，进而抑制流感病毒感染引起的炎症因子分泌，通过凋亡和自噬最终调节流感病毒的发病机制。
Background: Platycodin D (PLD) comes from the main triterpenoid saponins in the dry roots of Platycodon grandiflorum, which has been used in traditional Chinese medicine in the treatment of respiratory disease. This study aimed to evaluate the therapeutic effects of PLD on mouse alveolar macrophages induced by influenza A virus (IAV) in vitro and to investigate the PLD of its action. Materials and Methods: Raw264.7 mouse alveolar macrophages were grown in culture and the Cell Counting Kit-8 assay determined cell viability. Cells were treated for 24 h with increasing doses of PLD (0, 5, 10, 20, 40, 60, 80, 100 μM). The apoptosis was detected by Annexin V-FITC/PI and Tunel assay. Levels of interleukin (IL)-1 β, IL-6 and tumor necrosis factor (TNF)-α of supernatants were assessed using enzyme-linked immunosorbent assay. The numbers of phagophores, autophagosomes, and autolysosomes and the ultrastructure of cells were assessed using transmission electron microscope assay. The relative protein expression of microtubule-associated protein light chain (LC) 3I, LC3II, Beclin-1, and B cell lymphoma (Bcl2) was evaluated by Western blotting. The localizations of Beclin-1 and Bcl2 were viewed with a fluorescence microscope. Results: PLD treatment resulted in significant dose-dependent inhibition of the growth of Raw264.7 cells with the half-maximal inhibitory concentration of 115.74 μM at 24 h. After Raw264.7 cells infected by influenza virus, PLD (2.5, 5, and 10 μM) can improve the activity of cells, reducing apoptosis of cells, decreasing the concentrations of IL-1 β, IL-6, and TNF-α in the supernatant of cells, reducing autophagic injury of cells and regulating the protein expression of Beclin-1 and Bcl2. Conclusion: PLD could inhibit excessive apoptosis and autophagy of macrophage cells induced by IAV in a dose-dependent manner and then inhibits the secretion of inflammatory factors caused by influenza virus infection caused by apoptosis and autophagy and then ultimately regulate the pathogenesis of influenza virus.
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影响因子：
--
作者：
Zhang R;Chi X;Wang S;Qi B;Yu X;Chen JL
通讯作者：
Chen JL
影响因子：
10
作者：
Bhuiyan MU;Snelling TL;West R;Lang J;Rahman T;Granland C;de Gier C;Borland ML;Thornton RB;Kirkham LS;Sikazwe C;Martin AC;Richmond PC;Smith DW;Jaffe A;Blyth CC
通讯作者：
Blyth CC
Platycodin D 抑制原代细胞系和已建立细胞系中的 2 型猪繁殖与呼吸综合征病毒
DOI：
10.3390/v10110657
发表时间：
2018-11-21
期刊：
Viruses
影响因子：
--
作者：
Zhang M;Du T;Long F;Yang X;Sun Y;Duan M;Zhang G;Liu Y;Zhou EM;Chen W;Chen J
通讯作者：
Chen J
从Anemarrhena Asphodeloides的根茎分离的化合物的抗炎活性。
DOI：
10.3390/molecules23102631
发表时间：
2018-10-13
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Wang Z;Cai J;Fu Q;Cheng L;Wu L;Zhang W;Zhang Y;Jin Y;Zhang C
通讯作者：
Zhang C
影响因子：
30.3
作者：
Beale R;Wise H;Stuart A;Ravenhill BJ;Digard P;Randow F
通讯作者：
Randow F