Prophages encode phage-defense systems with cognate self-immunity.
Prophages encode phage-defense systems with cognate self-immunity.
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DOI：
10.1016/j.chom.2021.09.002
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发表时间：
2021-11-10
影响因子：
30.3
通讯作者：
Hinton JCD
中科院分区：
文献类型：
--
作者：
Owen SV;Wenner N;Dulberger CL;Rodwell EV;Bowers-Barnard A;Quinones-Olvera N;Rigden DJ;Rubin EJ;Garner EC;Baym M;Hinton JCD
温带细菌普遍存在于细菌基因组中，作为垂直遗传的岛屿存在，称为原噬菌体。益生菌易受外源性益生菌对其宿主细菌的捕食。在这里，我们确定BstA，一个家庭的原噬菌体编码的噬菌体防御蛋白在不同的革兰氏阴性细菌。BstA定位于外源噬菌体DNA复制的位点，介导流产感染，抑制竞争性噬菌体流行。在裂解复制期间，BstA编码原噬菌体本身不受BstA抑制，这是由于抗BstA（阿坝）元件（bstA基因座内的一段短DNA）赋予的自身免疫性。来自沙门氏菌、克雷伯氏菌和埃希氏菌的不同BstA蛋白对噬菌体复制的抑制作用通常是可互换的，但每种蛋白都具有同源的阿坝元件。阿坝元件的特异性确保了免疫力仅限于复制原噬菌体，防止了BstA编码变体的利用。BstA蛋白允许原噬菌体保护宿主细胞免受外源噬菌体攻击，而不牺牲裂解复制的能力。BstA是在革兰氏阴性阿坝的原噬菌体中发现的败育感染蛋白aba，bstA基因座内的短DNA序列，作为自身免疫元件阿坝赋予编码BstA的原噬菌体对BstA驱动的败育感染的免疫性。Owen等人表明这些蛋白质中的一些含有内部自身免疫系统，使得原噬菌体不会被其自身的噬菌体防御蛋白自我靶向。
Temperate phages are pervasive in bacterial genomes, existing as vertically inherited islands termed prophages. Prophages are vulnerable to predation of their host bacterium by exogenous phages. Here, we identify BstA, a family of prophage-encoded phage-defense proteins in diverse Gram-negative bacteria. BstA localizes to sites of exogenous phage DNA replication and mediates abortive infection, suppressing the competing phage epidemic. During lytic replication, the BstA-encoding prophage is not itself inhibited by BstA due to self-immunity conferred by the anti-BstA (aba) element, a short stretch of DNA within the bstA locus. Inhibition of phage replication by distinct BstA proteins from Salmonella, Klebsiella, and Escherichia prophages is generally interchangeable, but each possesses a cognate aba element. The specificity of the aba element ensures that immunity is exclusive to the replicating prophage, preventing exploitation by variant BstA-encoding phages. The BstA protein allows prophages to defend host cells against exogenous phage attack without sacrificing the ability to replicate lytically. BstA is an abortive infection protein found in prophages of Gram-negative bacteria aba, a short DNA sequence within the bstA locus, acts as a self-immunity element aba gives BstA-encoding prophages immunity to BstA-driven abortive infection Variant BstA proteins have distinct and cognate aba elements Prophages can encode abortive infection proteins that give their bacterial hosts population-level phage defense. Owen et al. show that some of these proteins contain internal self-immunity systems so that the prophage is not self-targeted by its own phage-defense protein.
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影响因子：
3.5
作者：
CHEREPANOV, PP;WACKERNAGEL, W
通讯作者：
WACKERNAGEL, W
影响因子：
--
作者：
Green R;Rogers EJ
通讯作者：
Rogers EJ
预言对细菌病原体的进化和毒力的重要性。
影响因子：
5.2
作者：
Fortier LC;Sekulovic O
通讯作者：
Sekulovic O
影响因子：
14.9
作者：
Buchan, Daniel W. A.;Jones, David T.
通讯作者：
Jones, David T.
影响因子：
2.5
作者：
Capek, Martin;Janacek, Jiri;Kubinova, Lucie
通讯作者：
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