Preparation and evaluation of chitosan-polyvinyl alcohol/polyhexamethylene guanidine hydrochloride antibacterial dressing to accelerate wound healing for infectious skin repair.
Preparation and evaluation of chitosan-polyvinyl alcohol/polyhexamethylene guanidine hydrochloride antibacterial dressing to accelerate wound healing for infectious skin repair.
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壳聚糖-聚乙烯醇/盐酸聚六亚甲基胍抗菌敷料加速伤口愈合感染性皮肤修复的制备及评价
DOI：
10.21037/atm-21-509
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发表时间：
2021-03
影响因子：
--
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Yue X;Liu L;Wu Y;Liu X;Li S;Zhang Z;Han S;Wang X;Chang Y;Bai H;Chai J;Hu S;Wang H
背景伤口感染，尤其是多药耐药（MDR）细菌感染，是临床医学面临的主要挑战。方法以壳聚糖-聚乙烯醇（CTS-PVA）为乳化剂，加入盐酸聚胍（PHMG），采用冻干技术制备抗菌海绵。CTS-PVA/PHMG海绵对金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、白色念珠菌、耐甲氧西林金黄色葡萄球菌、多重耐药铜绿假单胞菌和多重耐药鲍曼不动杆菌的体外抗菌能力。并对其结构和物理性能进行了表征。在铜绿假单胞菌感染的全层小鼠皮肤伤口缺损模型中测试海绵敷料。通过创面面积测量和组织学分析评价疗效。结果CTS-PVA/PHMG海绵具有广谱抗菌能力，包括对临床来源的MDR细菌菌株的抗菌能力，同时保持良好的理化性能，包括高溶胀度和良好的保湿能力。扫描电子显微镜图像显示了CTS-PVA/PHMG海绵敷料的表面形态。创面愈合率检测和组织学分析支持新型敷料能够减轻炎症，加快感染创面和体内愈合速度。结论CTS-PVA/PHMG海绵具有广谱抗菌活性，可为临床防治超级细菌感染创面提供新的途径。
Background Wound infections, especially multidrug-resistant (MDR) bacterial infections, are a major challenge in clinical medicine. Methods In this study, a new type of antibacterial sponge was prepared from a solution containing a chitosan-polyvinyl alcohol (CTS-PVA) emulsion with added polyhexamethylene guanidine hydrochloride (PHMG) in a homogeneous medium using lyophilization technology. The antibacterial ability of and CTS-PVA/PHMG sponge against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Methicillin-resistant Staphylococcus aureus, multidrug-resistant Pseudomonas aeruginosa, and multidrug-resistant Acinetobacter baumannii in vitro. The structure and physical properties were characterized. The sponge dressing was tested in a Pseudomonas aeruginosa-infected full-thickness mouse skin wound defect model. The effects were evaluated by wound area measurement and histological analysis. Results The CTS-PVA/PHMG sponge showed broad-spectrum antibacterial ability, including for MDR bacterial stains from clinical sources, while maintaining excellent physicochemical properties, including a high swelling degree and good moisture retention capability. Scanning electron microscopy images displayed the surface morphology of the CTS-PVA/PHMG sponge dressing. The detection of the wound healing rate and histological analysis supported that the new dressing can alleviate the inflammation and accelerate the healing speed of infected wounds and in vivo. Conclusions CTS-PVA/PHMG sponge shows broad-spectrum antibacterial activity, which can provide a new pathway for clinical prevention and treatment of superbug-infected wounds.
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影响因子：
4
作者：
Shi Q;Qian Z;Liu D;Sun J;Wang X;Liu H;Xu J;Guo X
通讯作者：
Guo X
影响因子：
10.7
作者：
Kamoun EA;Kenawy ES;Chen X
通讯作者：
Chen X
影响因子：
2.9
作者：
Mauldin, Patrick D.;Salgado, Cassandra D.;Bosso, John A.
通讯作者：
Bosso, John A.
影响因子：
9.7
作者：
Monteiro, Nelson;Martins, Margarida;Neves, Nuno M.
通讯作者：
Neves, Nuno M.
DOI：
10.3390/molecules21070836
发表时间：
2016-06-27
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Rudramurthy GR;Swamy MK;Sinniah UR;Ghasemzadeh A
通讯作者：
Ghasemzadeh A